Lamers J M, Dekkers D H, Bezstarosti K, Meij J T, van Heugten H A
Department of Biochemistry, Faculty of Medicine and Health Sciences, Erasmus University Rotterdam, The Netherlands.
Mol Cell Biochem. 1992 Oct 21;116(1-2):59-67. doi: 10.1007/BF01270570.
In the last decade a great deal of attention was awarded to a signal transduction pathway which is utilized primarily by 'Ca2+ mobilizing' signal molecules and which involves the hydrolysis of a quantitatively minor phospholipid, phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) by a PtdIns-specific phospholipase C (PLC). The evidence for the existence of receptor-mediated GTP binding protein-coupled PLC in myocardium and its possible functions are briefly summarized. The minireview is concentrated on the following aspects: 1) cellular localization and synthesis of polyphospho-PtdIns from PtdIns, 2) desensitization of the alpha 1-adrenergic agonist and endothelin-1 mediated PtdIns responses, 3) oscillatory Ca2+ transients initiated by PtdIns(4,5)P2 hydrolysis, 4) polyunsaturated fatty acids as constituents of polyphospho-PtdIns and of the protein kinase C activator 1,2-diacylglycerol (DAG), 5) source other than PtdIns(4,5)P2 contributing to the stimulated DAG, 6) role of the PtdIns pathway in cardiomyocyte growth and gene expression during the hypertrophic response.
在过去十年中，一条信号转导途径受到了极大关注，该途径主要被“Ca2+动员”信号分子所利用，涉及一种定量上较少的磷脂——磷脂酰肌醇4,5-二磷酸（PtdIns(4,5)P2）被一种PtdIns特异性磷脂酶C（PLC）水解。简要总结了心肌中受体介导的GTP结合蛋白偶联PLC存在的证据及其可能的功能。这篇小型综述集中在以下几个方面：1）从PtdIns合成多磷酸-PtdIns的细胞定位和合成，2）α1-肾上腺素能激动剂和内皮素-1介导的PtdIns反应的脱敏，3）由PtdIns(4,5)P2水解引发的振荡性Ca2+瞬变，4）多不饱和脂肪酸作为多磷酸-PtdIns和蛋白激酶C激活剂1,2-二酰基甘油（DAG）的组成成分，5）除PtdIns(4,5)P2之外对刺激的DAG有贡献的来源，6）PtdIns途径在肥厚反应期间心肌细胞生长和基因表达中的作用。
