Geary W A, Cooper P H
Department of Pathology, University of Virginia Health Sciences Center, Charlottesville 22908.
J Cutan Pathol. 1992 Dec;19(6):458-68. doi: 10.1111/j.1600-0560.1992.tb01598.x.
A commercially available antibody to proliferating cell nuclear antigen was used to characterize and compare proliferating cell populations in paraffin sections of benign, premalignant, and malignant lesions of human epidermis using routine immunohistochemical techniques. Three patterns emerged. An ordered pattern was found in prurigo nodularis and keratoacanthoma, wherein moderately and strongly positive nuclei were distributed in a continuous, basal-suprabasal layer of relatively uniform thickness. There was graded loss and ultimate extinction of PCNA staining in progressively more superficial epidermal cells. A basal dysplastic pattern was found in actinic keratosis and squamous cell carcinoma. Nuclei of essentially all dysplastic cells of both categories expressed PCNA, with a preponderance of strongly positive nuclei. These were localized to basal-suprabasal zones that were often expanded. Loss of PCNA reactivity toward the surface was often abrupt. Bowen's disease exhibited a diffuse dysplastic pattern, wherein large numbers of moderately and strongly positive nuclei, in random array, were present in essentially full thickness distribution. In many fields, however, a layer of cytologically bland basal cells, with faint or no nuclear staining, was interposed between dysplastic epithelium and dermis. This study has demonstrated that proliferating cell populations in epidermal lesions can be assessed with simple, inexpensive methods. There were consistent differences between the proliferating cell populations of the various entities studied, differences that can be reasonably correlated with other known clinical, microscopic, and biologic features of the lesions. This technique should provide an interesting new avenue for study of diverse cutaneous diseases.
利用常规免疫组织化学技术，采用一种市售的增殖细胞核抗原抗体，对人表皮良性、癌前和恶性病变石蜡切片中的增殖细胞群进行表征和比较。出现了三种模式。在结节性痒疹和角化棘皮瘤中发现一种有序模式，其中中等强度和强阳性细胞核分布在相对均匀厚度的连续基底层和基底上层。在逐渐浅表的表皮细胞中，PCNA染色逐渐减少并最终消失。在光化性角化病和鳞状细胞癌中发现一种基底发育异常模式。这两类所有发育异常细胞的细胞核均表达PCNA，强阳性细胞核占优势。它们定位于常扩张的基底层和基底上层区域。PCNA对表面的反应性丧失通常很突然。鲍恩病表现出弥漫性发育异常模式，其中大量中等强度和强阳性细胞核随机排列，基本呈全层分布。然而，在许多区域，在发育异常上皮和真皮之间有一层细胞学上平淡的基底细胞，核染色微弱或无染色。本研究表明，可用简单、廉价的方法评估表皮病变中的增殖细胞群。在所研究的各种实体的增殖细胞群之间存在一致的差异，这些差异可合理地与病变的其他已知临床、显微镜和生物学特征相关联。这项技术应为多种皮肤疾病的研究提供一条有趣的新途径。
