Ohmori M, Endo T, Ikeda M, Onaya T
Third Department of Internal Medicine, University of Yamanashi Medical School, Japan.
J Endocrinol. 1992 Dec;135(3):479-84. doi: 10.1677/joe.0.1350479.
Eight rabbits were immunized with a synthetic peptide corresponding to the unique N-terminal region (termed N peptide; amino acid residues 29-57) in the extracellular domain of the human thyrotrophin (TSH) receptor. After 10 weeks, all of the eight rabbits produced anti-N peptide antibodies. Western blot analysis revealed that the antibodies recognized rabbit TSH receptor as an approximately 100 kDa protein. We compared the level of thyroid hormone in serum taken before immunization (preimmune sera) with that of serum taken after immunization (postimmune sera) in these immunized rabbits. Postimmune sera from the eight rabbits had higher mean (+/- S.D.) levels of tri-iodothyronine (T3) and thyroxine (T4) than did preimmune sera (T3, preimmune 0.82 +/- 0.26 micrograms/l vs postimmune 1.33 +/- 0.35, P < 0.01; T4, preimmune 33.7 +/- 10.0 micrograms/l vs postimmune 41.0 +/- 6.0, P < 0.05). T3 levels in four rabbits and T4 levels in four rabbits after immunization were over the normal range obtained from six age-matched control rabbits. Seven rabbits exhibited thyroid-stimulating antibody (TSAb) activity with various degrees (241-545%). The concentration of T3 and T4 did not increase over 10 weeks in either non-immunized rabbits (T3, preimmune 0.89 +/- 0.34 micrograms/l vs postimmune 0.82 +/- 0.22; T4, preimmune 31.1 +/- 7.3 micrograms/l vs postimmune 30.3 +/- 5.1) or other peptide-immunized rabbits (T3, preimmune 0.68 micrograms/l (n = 2) vs postimmune 0.69; T4, preimmune 33.1 micrograms/l vs postimmune 26.4). These results indicate that experimentally produced anti-TSH receptor antibody with TSAb activity induces an increase in thyroid hormone in rabbits.
用与人类促甲状腺激素（TSH）受体胞外域独特的N端区域相对应的合成肽（称为N肽；氨基酸残基29 - 57）对8只兔子进行免疫。10周后，所有8只兔子均产生了抗N肽抗体。蛋白质印迹分析显示，这些抗体将兔TSH受体识别为一种约100 kDa的蛋白质。我们比较了这些免疫兔子免疫前采集的血清（免疫前血清）和免疫后采集的血清（免疫后血清）中的甲状腺激素水平。8只兔子的免疫后血清中三碘甲状腺原氨酸（T3）和甲状腺素（T4）的平均（±标准差）水平高于免疫前血清（T3，免疫前0.82±0.26微克/升，免疫后1.33±0.35，P < 0.01；T4，免疫前33.7±10.0微克/升，免疫后41.0±6.0，P < 0.05）。免疫后4只兔子的T3水平和4只兔子的T4水平超过了从6只年龄匹配的对照兔子获得的正常范围。7只兔子表现出不同程度（241 - 545%）的促甲状腺素受体抗体（TSAb）活性。在未免疫的兔子（T3，免疫前0.89±0.34微克/升，免疫后0.82±0.22；T4，免疫前31.1±7.3微克/升，免疫后30.3±5.1）或其他肽免疫的兔子（T3，免疫前0.68微克/升（n = 2），免疫后0.69；T4，免疫前33.1微克/升，免疫后26.4）中，T3和T4的浓度在10周内均未升高。这些结果表明，实验产生的具有TSAb活性的抗TSH受体抗体可诱导兔子甲状腺激素水平升高。
