Rose M D, Shearer G
Ministry of Agriculture, Fisheries and Food, Food Science Laboratory, Norwich, UK.
J Chromatogr. 1992 Oct 30;624(1-2):471-7. doi: 10.1016/0021-9673(92)85696-q.
A multi-residue method for the determination of tranquiliser residues in animal tissue is described. The procedure may be used to determine residues of the tranquilisers acepromazine, azaperone, chlorpromazine, haloperidol, propionylpromazine, xylazine, the metabolite of azaperone, azaperol, and the beta-adrenoreceptor blocking agent carazolol. Existing methods of analysis for tranquilisers are based on ultraviolet and fluorescence detection and have been used for pig kidney analysis. Determination in this method was by high-performance liquid chromatography with electrochemical detection in the screen mode. The enhanced selectivity offered by the electrochemical detector allowed determination in liver extracts, which often give rise to more interferences on chromatographic traces when using conventional methods of detection. The method offers up to a ten-fold improvement in limits of determination over methods using ultraviolet and fluorescence detection. Recoveries and coefficients of variation have been determined in the range 2-25 micrograms/kg in pig kidney and liver. This electrochemical detection method has been used to measure residues in routine surveillance programmes.
本文描述了一种用于测定动物组织中镇静剂残留量的多残留方法。该程序可用于测定乙酰丙嗪、阿扎哌隆、氯丙嗪、氟哌啶醇、丙酰丙嗪、赛拉嗪、阿扎哌隆的代谢产物阿扎哌醇以及β-肾上腺素受体阻滞剂卡拉洛尔等镇静剂的残留量。现有的镇静剂分析方法基于紫外和荧光检测，已用于猪肾分析。本方法采用高效液相色谱法，在筛选模式下进行电化学检测。电化学检测器提供的增强选择性使得能够在肝脏提取物中进行测定，而使用传统检测方法时，肝脏提取物在色谱图上往往会产生更多干扰。与使用紫外和荧光检测的方法相比，该方法的测定限提高了多达10倍。已在猪肾和肝脏中测定了2 - 25微克/千克范围内的回收率和变异系数。这种电化学检测方法已用于常规监测计划中的残留量测定。
