Mendiola M V, Martín C, Otal I, Gicquel B
Unité Génie Microbiologique (URA 1300 du CNRS), Institut Pasteur, Paris.
Res Microbiol. 1992 Oct;143(8):767-72. doi: 10.1016/0923-2508(92)90104-v.
A high degree of IS6110 restriction fragment length polymorphism (RFLP) is observed amongst the different strains of the Mycobacterium tuberculosis complex. The sequences of the IS6110 flanking regions from a M. tuberculosis strain harbouring four IS6110 copies were determined. Duplication of 3-4 nucleotides was found at the extremities of the four IS6110 copies, suggesting that IS6110 RFLP is due to transposition of the IS element. One of the copies of IS6110 analysed in the study was shown to be located at the same site in the genome of M. tuberculosis as the single copy present in an M. bovis BCG strain.
在结核分枝杆菌复合群的不同菌株中观察到高度的IS6110限制性片段长度多态性（RFLP）。测定了携带四个IS6110拷贝的结核分枝杆菌菌株中IS6110侧翼区域的序列。在四个IS6110拷贝的末端发现了3 - 4个核苷酸的重复，这表明IS6110 RFLP是由于该IS元件的转座所致。该研究中分析的一个IS6110拷贝被证明与牛分枝杆菌卡介苗菌株中存在的单拷贝位于结核分枝杆菌基因组的同一位置。
