IS6110：结核分枝杆菌复合群中的序列保守性及其在DNA指纹图谱分析中的应用

IS6110: conservation of sequence in the Mycobacterium tuberculosis complex and its utilization in DNA fingerprinting.

作者信息

Cave M D, Eisenach K D, McDermott P F, Bates J H, Crawford J T

机构信息

Department of Anatomy, University of Arkansas for Medical Sciences, Little Rock.

出版信息

Mol Cell Probes. 1991 Feb;5(1):73-80. doi: 10.1016/0890-8508(91)90040-q.

DOI:10.1016/0890-8508(91)90040-q

PMID:1673228

Abstract

Multiple copies of an insertion sequence, IS6110, were shown to be present in the genome of members of the Mycobacterium tuberculosis complex (M. tuberculosis and M. bovis). Ten to 12 copies are present in various strains of M. tuberculosis, while strains of M. bovis contain only one to three copies. IS6110 was not detected in the DNA of other species of mycobacteria. Restriction endonuclease analysis indicated that the sequence of IS6110 is conserved across strain and species lines. Hybridization to the insertion sequence can be used to detect restriction fragment length polymorphism reflecting divergence in the sequence of regions flanking the various copies of IS6110. These differences were used to fingerprint various strains of the M. tuberculosis complex.

摘要

研究表明，结核分枝杆菌复合群（结核分枝杆菌和牛分枝杆菌）成员的基因组中存在多个插入序列IS6110拷贝。结核分枝杆菌的不同菌株中存在10至12个拷贝，而牛分枝杆菌菌株仅含有1至3个拷贝。在其他分枝杆菌物种的DNA中未检测到IS6110。限制性内切酶分析表明，IS6110的序列在菌株和物种间是保守的。与插入序列的杂交可用于检测限制性片段长度多态性，这反映了IS6110各个拷贝侧翼区域序列的差异。这些差异被用于对结核分枝杆菌复合群的不同菌株进行指纹识别。