Perks A M, Kwok Y N, McIntosh C H, Ruiz T, Kindler P M
Department of Zoology, University of British Columbia, Vancouver, Canada.
J Dev Physiol. 1992 Oct;18(4):151-9.
Somatostatin-like immunoreactivity was measured by radioimmunoassay with a monoclonal antibody in lungs from perinatal guinea pigs (62 +/- 2 days of gestation). Fetuses delivered by Caesarean section and dissected before breathing showed 4748 +/- 758 pg/lung (n = 25). Fetuses allowed to breathe (neonates) showed marked increases in activity: 7629 +/- 1355 pg/lung (n = 12) after breathing 30 seconds, and 10729 +/- 1064 pg/lung (n = 6) after breathing 3 minutes (2.3-fold increase, P < 0.005). Values then declined (5203 +/- 1050 pg/lung (n = 9) at 30 minutes; 1458 +/- 105 pg/lung (n = 4) at 60 minutes). Changes were similar in pg/g wet tissue. HPLC characterized the immunoreactive peptides as somatostatin-14 (SS-14) and somatostatin-28 (SS-28) in both fetuses and neonates (n = 11). SS-28 made up only 13.7 +/- 1.7% of the activity; this percentage did not change with breathing. The effects of synthetic SS-14 on lung liquid production were investigated in in vitro lungs from 42 fetal guinea pigs. All 21 preparations immersed in 10(-5)-10(-7) M SS-14 during the middle hour of 3 h incubations reduced production, often approaching zero after treatment (rates, ml/kg body weight per h, succeeding hours: 10(-5) M (n = 9), 3.09 +/- 0.68, 0.93 +/- 0.39, -0.05 +/- 0.60 (fall significant during and after treatment, P < 0.025-0.005); 10(-6) M (n = 6), 3.06 +/- 0.68, 1.29 +/- 0.58, 0.36 +/- 0.38 (P < 0.05-0.005); 10(-7) M (n = 6), 1.96 +/- 0.66, 1.11 +/- 0.34, 0.64 +/- 0.28 (P < 0.05-0.025).(ABSTRACT TRUNCATED AT 250 WORDS)
采用放射免疫分析法，使用单克隆抗体测定围产期豚鼠（妊娠62±2天）肺组织中的生长抑素样免疫反应性。剖宫产取出并在呼吸前解剖的胎儿肺组织中生长抑素样免疫反应性为4748±758 pg/肺（n = 25）。允许呼吸的胎儿（新生儿）的活性显著增加：呼吸30秒后为7629±1355 pg/肺（n = 12），呼吸3分钟后为10729±1064 pg/肺（n = 6）（增加2.3倍，P < 0.005）。随后该值下降（30分钟时为5203±1050 pg/肺（n = 9）；60分钟时为1458±105 pg/肺（n = 4））。以pg/g湿组织计，变化情况相似。高效液相色谱法鉴定出胎儿和新生儿（n = 11）肺组织中的免疫反应性肽为生长抑素-14（SS-14）和生长抑素-28（SS-28）。SS-28仅占活性的13.7±1.7%；该百分比不随呼吸而变化。在42只豚鼠胎儿的离体肺组织中研究了合成SS-14对肺液生成的影响。在3小时孵育的中间1小时，所有21份标本浸入10⁻⁵ - 10⁻⁷ M SS-14中，肺液生成均减少，处理后常接近零（每小时每千克体重的生成速率，后续小时：10⁻⁵ M（n = 9），3.09±0.68，0.93±0.39，-0.05±0.60（处理期间及处理后下降显著，P < 0.025 - 0.005）；10⁻⁶ M（n = 6），3.06±0.68，1.29±0.58，0.36±0.38（P < 0.05 - 0.005）；10⁻⁷ M（n = 6），1.96±0.66，1.11±0.34，0.64±0.28（P < 0.05 - 0.025）。（摘要截短于250字）
