Jagielski M, Sztabińska-Koncka H, Rastawicki W, Szych J, Kałuzewski S, Mrozowska A
Zakład Bakteriologii PZH w Warszawie.
Med Dosw Mikrobiol. 1992;44(3-4):97-107.
This study was aimed at recognition of frequency of occurrence of P fimbriae in strains of Escherichia coli isolated from samples of feces of children with symptoms of diarrhoea and at search of these adhesions in strains representing other than Escherichia genera of Enterobacteriaceae strains. One hundred forty laboratory strains were investigated. They belonged to genus Citrobacter, Enterobacter, Hafnia, Klebsiella, Morganella, Proteus, Providencia, Salmonella, Shigella, and Yersinia. Also were tested 1277 colonies of enteric rods isolated from the MacConkey's medium inoculated with samples of feces from 163 children with symptoms of diarrhoea. Mannose-resistant active hemagglutination test was performed with human group O erythrocytes and guinea pig erythrocytes stabilized with glutaraldehyde. Presence of P fimbriae was detected by the slide latex test with latex covered by P1 glycoprotein. Among 140 laboratory strains of Enterobacteriaceae in 21 strains (3-E. cloaceae, 2-Hafnia, 13-K. pneumoniae, 2-P. rettgeri and in one strains of Providencia) presence of MRHA adhesins was demonstrated. Nine of these strains (2-Hafnia, 5-K. pneumoniae and 2-P. rettgeri) reacted specifically in the latex test. Among 1142 colonies of E. coli isolated from children with symptoms of diarrhoea, 326 colonies belonged to 13 EPEC serotypes. With 118 (36.2%) of EPEC colonies a positive result of MRHA reaction was found with human erythrocytes and 34 (10.4%) with guinea pig erythrocytes. Positive latex test was obtained with 77 (23.6%) colonies. All these colonies possessed MRHA adhesins. Remaining 816 colonies of E. coli strains did not represent microorganisms belonging to serotypes accepted as enteropathogenic. From 112 (13.7%) colonies out of 816 not belonging to EPEC, positive results was obtained in the MRHA test with human erythrocytes and this was the case also with 41 (5.0%) colonies in MRHA reaction with application of guinea pig erythrocytes. The latex test was positive in 65 (7.9%) colonies of E. coli. From remaining 135 colonies other than E. coli, positive result of latex test of presence of P fimbriae was obtained with 54 (40.0%) colonies, including 14 colonies of E. cloacae, 23-K. pneumoniae and 17-K. oxytoca. In all these strains presence of MRHA adhesins was demonstrated. These investigations demonstrated that among EPEC strains significantly more frequently, than not belonging to these serotypes of E. coli, MRHA adhesins, including P fimbriae was observed.(ABSTRACT TRUNCATED AT 400 WORDS)
本研究旨在确定从有腹泻症状儿童的粪便样本中分离出的大肠杆菌菌株中P菌毛的出现频率，并在代表肠杆菌科中除大肠杆菌属以外的其他菌株中寻找这些黏附素。研究了140株实验室菌株。它们分别属于柠檬酸杆菌属、肠杆菌属、哈夫尼亚菌属、克雷伯菌属、摩根菌属、变形杆菌属、普罗威登斯菌属、沙门菌属、志贺菌属和耶尔森菌属。还对从接种了163名有腹泻症状儿童粪便样本的麦康凯培养基中分离出的1277个肠道杆菌菌落进行了检测。用人O型红细胞和经戊二醛稳定的豚鼠红细胞进行了甘露糖抗性活性血凝试验。通过用P1糖蛋白包被的乳胶进行玻片乳胶试验检测P菌毛的存在。在140株肠杆菌科实验室菌株中，有21株（3株阴沟肠杆菌、2株哈夫尼亚菌、13株肺炎克雷伯菌、2株雷氏普罗威登斯菌和1株普罗威登斯菌属菌株）表现出MRHA黏附素的存在。其中9株菌株（2株哈夫尼亚菌、5株肺炎克雷伯菌和2株雷氏普罗威登斯菌）在乳胶试验中呈特异性反应。在从有腹泻症状儿童中分离出的1142个大肠杆菌菌落中，326个菌落属于13种肠致病性大肠杆菌血清型。118个（36.2%）肠致病性大肠杆菌菌落与人红细胞的MRHA反应呈阳性，34个（10.4%）与豚鼠红细胞的反应呈阳性。77个（23.6%）菌落的乳胶试验呈阳性。所有这些菌落都具有MRHA黏附素。其余816个大肠杆菌菌株菌落不代表被认为是肠致病性的血清型微生物。在816个不属于肠致病性大肠杆菌的菌落中，有112个（13.7%）菌落与人红细胞的MRHA试验呈阳性结果，在应用豚鼠红细胞的MRHA反应中，41个（5.0%）菌落也是如此。65个（7.9%）大肠杆菌菌落的乳胶试验呈阳性。在除大肠杆菌以外的其余135个菌落中，54个（40.0%）菌落的P菌毛乳胶试验呈阳性结果，其中包括14个阴沟肠杆菌菌落、23个肺炎克雷伯菌菌落和17个产酸克雷伯菌菌落。在所有这些菌株中都证明了MRHA黏附素的存在。这些研究表明，在肠致病性大肠杆菌菌株中，观察到MRHA黏附素，包括P菌毛的频率明显高于不属于这些大肠杆菌血清型的菌株。（摘要截短至400字）
