Cobeljić M, Stojiljković I, Budisin A, Lepsanović Z, Lers N
Institute of Preventive Medicine, Military Medical Academy, Belgrade, Yugoslavia.
Epidemiol Infect. 1990 Feb;104(1):127-33. doi: 10.1017/s0950268800054601.
Stool specimens from 631 children with acute diarrhoea and from 277 healthy controls were tested for the presence of non-enteropathogenic, non-enterotoxigenic Escherichia coli strains which mediated mannose-resistant haemagglutination of human erythrocytes (MRHA+). Fifty-nine (34.9%) of 169 isolated MRHA+ strains but none of 210 MRHA- strains exhibited diffuse adherence (DA+) to HEp-2 cells. DA+ strains were found in 37 (5.9%) children with diarrhoea and in 22 (7.9%) controls. MRHA+/DA+ strains in comparison to MRHA+/DA- strains significantly less frequently expressed P fimbriae (10.7 vs. 73.6%), haemolysin production (12.5 vs. 63.2%), and MRHA of other species erythrocytes (21.4 vs. 84%). These data demonstrate that E. coli which exhibit the diffuse pattern of adherence to HEp-2 cells also cause MRHA of human erythrocytes. Since these strains were found with similar frequencies in children with and without diarrhoea it seems that DA is not a marker of enteropathogenicity of E. coli.
对631名急性腹泻儿童和277名健康对照儿童的粪便标本进行检测，以确定是否存在介导人红细胞甘露糖抗性血凝反应（MRHA+）的非致病性、非产肠毒素大肠杆菌菌株。169株分离出的MRHA+菌株中有59株（34.9%）对HEP-2细胞表现出弥散性黏附（DA+），而210株MRHA-菌株均未表现出这种情况。在37名（5.9%）腹泻儿童和22名（7.9%）对照儿童中发现了DA+菌株。与MRHA+/DA-菌株相比，MRHA+/DA+菌株表达P菌毛的频率显著较低（10.7%对73.6%），产生溶血素的频率显著较低（12.5%对63.2%），对其他物种红细胞的MRHA频率也显著较低（21.4%对84%）。这些数据表明，对HEP-2细胞表现出弥散性黏附模式的大肠杆菌也会引起人红细胞的MRHA。由于在腹泻儿童和非腹泻儿童中发现这些菌株的频率相似，因此似乎DA不是大肠杆菌致病性的标志物。
