Mathiesen T, Collins V P, Ohlsson E, Sundqvist V A, Wahren B
Department of Neurosurgery, Karolinska Institute, Stockholm, Sweden.
Acta Virol. 1992 Dec;36(6):551-6.
Productive infections with cytomegalovirus (CMV) and human immunodeficiency virus (HIV) were established in the Tp41ON cell line derived from a human esthesioneuroblastoma. HIV antigen expression was highest in cultures coinfected with CMV and HIV. Viral infection caused increased MHC class I antigen expression while class II and CD4 antigens remained undetectable using immunofluorescence methods. Uninfected cultures showed 10% and coinfected cultures 80% class I antigen positive cells. In coinfected cultures, CMV and HIV antigens were detected in 4% and 8% of the cells, respectively. The detection of CMV antigens in some multinucleated cells suggests coinfection with both viruses in these cells, as multinucleated cells were not found in cultures infected with CMV only. The study shows that a cell line showing neuronal differentiation in vitro can be infected with CMV and HIV and that this infection increases MHC class I antigen expression.
在源自人嗅神经母细胞瘤的Tp41ON细胞系中建立了巨细胞病毒（CMV）和人类免疫缺陷病毒（HIV）的增殖性感染。在CMV和HIV共感染的培养物中，HIV抗原表达最高。病毒感染导致MHC I类抗原表达增加，而使用免疫荧光方法未检测到II类和CD4抗原。未感染的培养物中10%的细胞I类抗原呈阳性，共感染的培养物中80%的细胞I类抗原呈阳性。在共感染的培养物中，分别在4%和8%的细胞中检测到CMV和HIV抗原。在一些多核细胞中检测到CMV抗原，这表明这些细胞同时感染了两种病毒，因为在仅感染CMV的培养物中未发现多核细胞。该研究表明，一种在体外显示神经元分化的细胞系可被CMV和HIV感染，且这种感染会增加MHC I类抗原表达。
