Isohata E, Toyoda M, Saito Y
Eisei Shikenjo Hokoku. 1990(108):104-9.
An analytical method for aflatoxins using solvent extraction, affinity column (containing monoclonal antibodies against aflatoxins bound onto agarose) clean-up and high performance liquid chromatographic determination was applied to the analysis of aflatoxins in peanuts, pistachio nuts, white and black peppers and corn. Recoveries of aflatoxins B1, B2, G1 and G2 spiked to peanuts, pistachio nuts and corn at the level of 20 ppb were 77.4-81.4, 70.1-77.9 and 72.3-94.8%, respectively. In the case of white and black peppers, because the spiked aflatoxin recovery rate was low, further purification steps should be studied. The stability of the affinity column was good. This procedure is recommended as a rapid and reliable method for the analysis of aflatoxins in the three kinds of foods, peanuts, pistachio nuts and corn.
采用溶剂萃取、亲和柱（含有结合在琼脂糖上的抗黄曲霉毒素单克隆抗体）净化和高效液相色谱测定的黄曲霉毒素分析方法，用于分析花生、开心果、白胡椒、黑胡椒和玉米中的黄曲霉毒素。在花生、开心果和玉米中添加水平为20 ppb的黄曲霉毒素B1、B2、G1和G2的回收率分别为77.4 - 81.4%、70.1 - 77.9%和72.3 - 94.8%。对于白胡椒和黑胡椒，由于添加黄曲霉毒素的回收率较低，应研究进一步的净化步骤。亲和柱的稳定性良好。该方法推荐作为花生、开心果和玉米这三种食品中黄曲霉毒素分析的快速可靠方法。
