Beebe R M
J Assoc Off Anal Chem. 1978 Nov;61(6):1347-52.
A method for determining aflatoxins by high pressure liquid chromatography (HPLC) with fluorescence detection after CB extraction and cleanup has been applied to various foods. Recoveries at 1--15 ppb levels from green coffee and peanut butter was 72--85 and 74--104%, respectively. Precision of the method has been tested for peanut butter. Other products to which the method has been successfully applied include tree nuts, seeds, grains, chocolate-covered peanut butter candy, and roasted, salted-in-shell peanuts. High levels of aflatoxins found in several samples of nuts by this method have been verified by the official thin layer chromatographic (TLC) method. The advantages of this HPLC method are speed, precision, sensitivity, selectivity, and immediate chemical confirmation of aflatoxins B1 and G1. None of the products analyzed required special cleanup procedures. Preparative-scale HPLC was used to isolate purified B1 for toxicity testing.
一种通过高压液相色谱（HPLC）结合荧光检测来测定黄曲霉毒素的方法，该方法在对样品进行提取和净化后已应用于各种食品。在1至15 ppb水平下，生咖啡豆和花生酱的回收率分别为72%至85%和74%至104%。该方法对花生酱的精密度进行了测试。该方法已成功应用的其他产品包括坚果、种子、谷物、巧克力包裹的花生酱糖果以及带壳烤盐渍花生。通过该方法在多个坚果样品中发现的高水平黄曲霉毒素已通过官方薄层色谱（TLC）方法得到验证。这种HPLC方法的优点是速度快、精密度高、灵敏度高、选择性好，并且能立即对黄曲霉毒素B1和G1进行化学确认。所分析的产品均无需特殊的净化程序。制备规模的HPLC用于分离纯化B1以进行毒性测试。
