Yamada T
Eisei Shikenjo Hokoku. 1990(108):109-11.
Simple and rapid determination method for butylated hydroxyanisole [2(3)-tert-butyl-4-hydroxyanisole)] (BHA) (CAS no. 25013-16-5) is developed. BHA was extracted from oily foods with hexane and from hexane layer to acetonitrile. The acetonitrile layer was either directly or after concentration injected to high-performance liquid chromatograph (HPLC) equipped with a fluorescence detector. As the internal standard for determination, tert-butyl-p-cresol was used. The conditions for HPLC were as follows. Column; Shim-pack CLC-ODS (6 mm i.d. x 15 cm): Mobile phase; 75% methanol: Excitation wave length for the fluorescence detector 298 nm; Emission wave length 332 nm. The recoveries of BHA from salad oil, margarine, and butter added 10 micrograms/g or 200 ng/g of BHA were satisfactory.
开发了一种简单快速的丁基羟基茴香醚2(3)-叔丁基-4-羟基茴香醚(CAS编号：25013-16-5)测定方法。BHA先用己烷从油性食品中萃取，再从己烷层萃取至乙腈中。乙腈层可直接注入或浓缩后注入配备荧光检测器的高效液相色谱仪(HPLC)中。以叔丁基对甲酚作为测定的内标物。HPLC的条件如下：色谱柱；岛津填充CLC-ODS(内径6mm×15cm)；流动相；75%甲醇；荧光检测器的激发波长298nm；发射波长332nm。从添加了10微克/克或200纳克/克BHA的色拉油、人造黄油和黄油中回收BHA的效果令人满意。
