Yankah V V, Ushio H, Ohshima T, Koizumi C
Department of Food Science and Technology, Tokyo University of Fisheries, Japan.
Lipids. 1998 Nov;33(11):1139-45. doi: 10.1007/s11745-998-0316-5.
Concentrations of synthetic antioxidants butylated hydroxyanisole, butylated hydroxytoluene, and tert-butyl hydroquinone were quantified using a high-performance liquid chromatograph with spectrofluorometric detector. The antioxidants were separated and eluted on a reversed-phase column by gradient of a mixture of H2O/acetonitrile/acetic acid (66.5: 28.5:5, by vol) and a mixture of acetonitrile/acetic acid (95:5, vol/vol). The eluants were monitored at emission and excitation wavelengths of 310 and 280 nm, respectively. Calibration curves obtained using peak areas against concentration showed high coefficients of multiple determination (R2 > 0.99) for all antioxidants. Known concentrations of added antioxidant standards were recoverable within 98-99% from oils and over 93% from mouse blood. This method requires minimum sample extraction and purification before analysis and provides a relatively high percentage recovery. The method has been applied successfully for the measurement of antioxidant concentrations in oils, dried foods, and biological fluids.
使用配备荧光检测器的高效液相色谱仪对合成抗氧化剂丁基羟基茴香醚、丁基羟基甲苯和叔丁基对苯二酚的浓度进行定量分析。抗氧化剂在反相柱上通过水/乙腈/乙酸（66.5:28.5:5，体积比）和乙腈/乙酸（95:5，体积比）的混合物梯度洗脱分离。洗脱液分别在发射波长310nm和激发波长280nm处进行监测。使用峰面积与浓度绘制的校准曲线显示，所有抗氧化剂的多重测定系数均较高（R2>0.99）。添加的抗氧化剂标准品已知浓度在油中的回收率为98-99%，在小鼠血液中的回收率超过93%。该方法在分析前所需的样品提取和纯化最少，回收率相对较高。该方法已成功应用于油类、干燥食品和生物流体中抗氧化剂浓度的测定。
