Suzuki S, Ogawa Y, Hirose A, Kaneko T, Kurokawa Y
Eisei Shikenjo Hokoku. 1991(109):115-8.
The objective of this study was to obtain the normal ranges of essential metals in male and female Slc: Wister rats aged 9 or 11 weeks. Concentration of 10 elements (Ca, Cu, Fe, K, Mg, Mn, Na, P, S and Zn) in the lung, liver, kidney and spleen of rats was determined by inductively coupled argon plasma atomic emission spectrometry (ICP). Each sample was digested with nitric acid and perchloric acid in a sealed double Teflon vessel with polypropylene jacket by heating with a microwave oven. The concentrations of Fe and Mn in the liver were different between males and females. The Fe level in the female was higher than that in the male, and Mn level in male was higher than that in female. The concentration of Fe in the spleen was different by sex and ago. The Fe level in female was 2.3 times higher than that in male, and that in rats of 11 weeks was 1.8 times higher than that of 9 weeks.
本研究的目的是获取9周龄或11周龄雄性和雌性Slc:Wister大鼠体内必需金属的正常范围。采用电感耦合氩等离子体原子发射光谱法（ICP）测定大鼠肺、肝、肾和脾中10种元素（钙、铜、铁、钾、镁、锰、钠、磷、硫和锌）的浓度。每个样品在带有聚丙烯外套的密封双层聚四氟乙烯容器中用硝酸和高氯酸消化，通过微波炉加热。肝脏中铁和锰的浓度在雄性和雌性之间存在差异。雌性大鼠的铁水平高于雄性，而雄性大鼠的锰水平高于雌性。脾脏中铁的浓度因性别和年龄而异。雌性大鼠的铁水平是雄性的2.3倍，11周龄大鼠的铁水平是9周龄大鼠的1.8倍。
