Shimamura Tadashi, Iijima Satoe, Hirayama Mitsuhiro, Iwashita Masato, Akiyama Shigeru, Takaku Yuichi, Yumoto Sakae
School of Allied Health Sciences, Kitasato University, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0373, Japan.
J Trace Elem Med Biol. 2013 Oct;27(4):286-94. doi: 10.1016/j.jtemb.2013.04.006. Epub 2013 Apr 17.
The concentrations of 22 major and trace elements in livers from rats aging from 5 to 113 weeks old were determined. The rats investigated were the same rats previously reported with respect to 29 elements in bones (femur) and 26 elements in kidneys. The samples were decomposed with high-purity nitric acid and hydrogen peroxide. Seven elements (Na, Mg, P, K, Ca, Fe and Zn) were determined by inductively coupled plasma atomic emission spectrometry (ICP-AES), and 15 elements (Mn, Co, Cu, As, Se, Rb, Sr, Mo, Cd, Sn, Sb, Cs, Ba, Pb and Bi) were determined by inductively coupled plasma mass spectrometry (ICP-MS). Analysis of variance (ANOVA) for age variations indicated that the concentrations of many elements, such as Mg, P, K, Mn, Fe, Cu, Zn, Sr, Mo and Cd, were almost constant across the ages of the rats with the exception of 5 weeks old (p > 0.05). Arsenic, Pb and Bi showed significant increasing trends, while Na and Co showed decreasing trends (p < 0.01). Selenium showed a decreasing trend except at the initial stage of 5-9 weeks old. Calcium, Rb, Sn, Sb, Cs and Ba showed significant age-related variations, but their patterns were not monotonic. The liver clearly contrasts with the kidneys, in which many elements showed significant age-related variations with increasing trends. The concentration ranges of Mg, P, K, Mn, Cu, Zn, and Mo were controlled within 15% across all ages of rats. The homeostasis of the aforementioned elements may be well established in the liver. The toxic elements, such as Cd, Pb and Bi, showed a narrow concentration range among age-matched rats.
测定了5至113周龄大鼠肝脏中22种主要和微量元素的浓度。所研究的大鼠与之前报道的同一批大鼠，之前已对其股骨中的29种元素和肾脏中的26种元素进行了研究。样品用高纯度硝酸和过氧化氢分解。采用电感耦合等离子体原子发射光谱法（ICP-AES）测定7种元素（Na、Mg、P、K、Ca、Fe和Zn），采用电感耦合等离子体质谱法（ICP-MS）测定15种元素（Mn、Co、Cu、As、Se、Rb、Sr、Mo、Cd、Sn、Sb、Cs、Ba、Pb和Bi）。年龄变化的方差分析（ANOVA）表明，除5周龄外，许多元素如Mg、P、K、Mn、Fe、Cu、Zn、Sr、Mo和Cd在大鼠各年龄段的浓度几乎恒定（p>0.05）。砷、Pb和Bi呈显著上升趋势，而Na和Co呈下降趋势（p<0.01）。硒除在5至9周龄的初始阶段外呈下降趋势。钙、Rb、Sn、Sb、Cs和Ba表现出与年龄显著相关的变化，但其模式并非单调。肝脏与肾脏形成明显对比，在肾脏中许多元素随着年龄增长呈现出与年龄显著相关的变化且呈上升趋势。Mg、P、K、Mn、Cu、Zn和Mo的浓度范围在所有年龄段的大鼠中均控制在15%以内。上述元素的内稳态可能在肝脏中建立良好。Cd、Pb和Bi等有毒元素在年龄匹配的大鼠中浓度范围较窄。
