Mirkova E, Khalkova Zh
Probl Khig. 1992;17:117-22.
The genotoxic activity of the microbial preparation bulmoscid "in vivo" for somatic cells of mammals is studied by micronucleus method in marrow of mice. The experiments are performed on inbred mice C57BL6 of both sexes at unrepeated oral introduction of the preparation in two doses: 2200 mg/kg-1 (2.64.10(10) cells kg-1), and 1100 mg/kg-1 (1.32.10(10) cells kg-1) presenting 80% and respectively 40% of the unrepeated maximum tolerable dose of "Bulmoscid" for mice (2750 mg/kg-1). The genotoxic index, frequency of micronuclear polychromatophilic erythrocytes (MPE) in marrow is determined in dynamics on the 24, 48 and 72 hour after the introduction of the preparation. The data of the performed cytogenetic analysis in marrow of C57BL6 mice of both sexes point out that the preparation "Bulmoscid" possesses no clastogenic activity for mammals. The data received are extrapolated also in direction of absence of genotoxic potential for germinative cells.
采用小鼠骨髓微核试验法，研究了微生物制剂布尔莫西德“体内”对哺乳动物体细胞的遗传毒性活性。对C57BL6近交系雌雄小鼠进行实验，以两种剂量单次经口给予该制剂：2200mg/kg-1（2.64×10(10)个细胞/kg-1）和1100mg/kg-1（1.32×10(10)个细胞/kg-1），分别相当于小鼠单次最大耐受剂量“布尔莫西德”（2750mg/kg-1）的80%和40%。在给予制剂后的24、48和72小时动态测定骨髓中微核嗜多染红细胞（MPE）的频率，即遗传毒性指数。对C57BL6雌雄小鼠骨髓进行的细胞遗传学分析数据表明，制剂“布尔莫西德”对哺乳动物无断裂活性。所得数据也外推至生殖细胞无遗传毒性潜力的方向。
