Almici C, Donnenberg A D, Rizzoli V
Department of Hematology, University of Parma, Italy.
J Hematother. 1992 Fall;1(3):279-88. doi: 10.1089/scd.1.1992.1.279.
Counterflow centrifugal elutriation (CCE) separates mixed cell populations into distinct subpopulations, on the basis of different sedimentation characteristics, without impairment of cell function or yield. The advantages of this technique are the high recovery and viability of fractionated cells, as well as the rapidity and the reproducibility of results. CCE alone, or in combination with other separation methods, can provide homogeneous populations of cells for further investigations. Recently, CCE was employed in clinical studies aimed at preventing graft-versus-host disease in bone marrow transplant recipients, by depleting lymphocytes prior to bone marrow infusion. This article reviews the principles of elutriation and describes the possible experimental and clinical applications of this technique, which seems suitable for both peripheral blood (PB) and bone marrow (BM) separation.
逆流离心淘析（CCE）基于不同的沉降特性，将混合细胞群体分离成不同的亚群，而不会损害细胞功能或产量。该技术的优点是分离出的细胞回收率高、活力强，以及结果的快速性和可重复性。单独使用CCE或与其他分离方法结合使用，可以提供均匀的细胞群体用于进一步研究。最近，CCE被用于临床研究，旨在通过在骨髓输注前清除淋巴细胞来预防骨髓移植受者的移植物抗宿主病。本文综述了淘析的原理，并描述了该技术可能的实验和临床应用，该技术似乎适用于外周血（PB）和骨髓（BM）的分离。
