BADER S
J Biophys Biochem Cytol. 1959 Mar 25;5(2):217-29. doi: 10.1083/jcb.5.2.217.
The amounts of DNA in interphase nuclei were compared with the amounts of DNA in metaphase and anaphase figures in Feulgen-stained tissue sections of 5 specimens of the human ovarian papillary serous adenocarcinoma. The relative amounts of DNA per cell were determined by cytophotometric measurements of interphase nuclei at a single wavelength and of mitotic figures by the two wavelength method. The 5 specimens conformed to the stem cell concept of cell proliferation since anaphase distributions of amounts of DNA were restricted to a narrow range of DNA values indicating the successful mitosis of a single cell type (stem cell) out of several cell types whose presence were suggested by the wide spread of interphase and metaphase values. In addition, the data indicated that, in some instances, only the amounts of DNA in anaphase figures can reliably identify the stem cell. Changes in the frequency of dividing cells having doubled amounts of DNA, and/or the presence of cells resulting from endoreduplication can distort the interphase distribution of amounts of DNA and thus give rise to a modal DNA interphase value which is not the same as the DNA value of the stem cell (anaphase figures).
对5例人卵巢乳头状浆液性腺癌的福尔根染色组织切片中，间期核的DNA含量与中期和后期细胞的DNA含量进行了比较。通过在单一波长下对间期核进行细胞光度测量，并采用双波长法对有丝分裂图像进行测量，确定了每个细胞的相对DNA含量。这5个标本符合细胞增殖的干细胞概念，因为后期DNA含量的分布局限于较窄的DNA值范围，表明在几种细胞类型中，只有一种单一细胞类型（干细胞）成功进行了有丝分裂，这一点可从间期和中期值的广泛分布得到暗示。此外，数据表明，在某些情况下，只有后期细胞的DNA含量才能可靠地识别干细胞。DNA含量加倍的分裂细胞频率的变化，和/或内复制产生的细胞的存在，会扭曲间期DNA含量的分布，从而产生一个与干细胞（后期细胞）的DNA值不同的间期DNA模态值。
