LITTAU V C
J Biophys Biochem Cytol. 1959 Mar 25;5(2):231-4. doi: 10.1083/jcb.5.2.231.
Deoxyribonucleic acid can be added back to protein sites from which the original nucleic acid, ribo- or deoxyribo-, is removed. If sections of frozen-substituted ovarian follicle cells of a leafhopper are first extracted by hot trichloracetic acid to remove nucleic acids and then immersed in a solution of a commerical preparation of deoxyribonucleic acid, the nucleic acid becomes attached to nuclear and cytoplasmic sites and can be rendered visible by the Feulgen reaction. The addition occurs in certain other tissues as well. The results are discussed in relation to biochemical and other cytochemical investigations of the nucleoprotein complex.
脱氧核糖核酸可以重新添加到蛋白质位点上,而这些位点上原来的核酸(核糖核酸或脱氧核糖核酸)已被去除。如果先将叶蝉冷冻替代的卵巢卵泡细胞切片用热三氯乙酸提取以去除核酸,然后浸入商业制备的脱氧核糖核酸溶液中,核酸就会附着在细胞核和细胞质位点上,并可通过福尔根反应使其可见。这种添加也发生在某些其他组织中。结合核蛋白复合物的生化和其他细胞化学研究对结果进行了讨论。