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Cytological evidence that both RNA and DNA may form a complex with the same protein.RNA和DNA都可能与同一种蛋白质形成复合物的细胞学证据。
J Biophys Biochem Cytol. 1959 Mar 25;5(2):231-4. doi: 10.1083/jcb.5.2.231.
2
Comparative effects of heat on chromatography and ultra-violet absorption of deoxyribo- or ribo-nucleic acids.热对脱氧核糖核酸或核糖核酸的色谱分析及紫外线吸收的比较影响
Nature. 1959 Jul 4;184:36-8. doi: 10.1038/184036a0.
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Nucleic acid and protein metabolism of barley seedlings. I. The parallelism between ribonucleic acid content and ribonuclease activity.大麦幼苗的核酸与蛋白质代谢。I.核糖核酸含量与核糖核酸酶活性之间的平行关系。
Biochim Biophys Acta. 1962 Jan 22;55:97-104. doi: 10.1016/0006-3002(62)90935-6.
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Nucleic acid and protein metabolism during the mitotic cycle in Vicia faba.蚕豆有丝分裂周期中的核酸与蛋白质代谢
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Quantitative cytochemical determination of desoxyribonucleic acid with the Feulgen nucleal reaction.用福尔根核反应进行脱氧核糖核酸的定量细胞化学测定。
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[On the nucleic acid content of malignant human tumors and their metastases. 3. The desoxyribonucleic acid and ribonucleic acid content of malignant human tumors and their metastases in comparison with normal tissues].[关于人类恶性肿瘤及其转移灶的核酸含量。3. 人类恶性肿瘤及其转移灶与正常组织相比的脱氧核糖核酸和核糖核酸含量]
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Cytoplasmic label following tritiated thymidine treatment of Allium cepa L. roots. Cytochemical and electron microscope study.用氚标记胸腺嘧啶核苷处理洋葱（Allium cepa L.）根后的细胞质标记。细胞化学与电子显微镜研究
J Cell Biol. 1968 Nov;39(2):264-85. doi: 10.1083/jcb.39.2.264.
2
[Studies on oocyte nuclei of amphibians and teleosts with pseudoisocyanine].[用假异氰蓝对两栖类和硬骨鱼类卵母细胞核的研究]
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本文引用的文献

1
A Selective Staining Method for the Basic Proteins of Cell Nuclei.一种细胞核碱性蛋白质的选择性染色方法。
Proc Natl Acad Sci U S A. 1953 Oct;39(10):991-9. doi: 10.1073/pnas.39.10.991.
2
The composition and structure of isolated chromosomes.分离染色体的组成与结构。
J Gen Physiol. 1951 May;34(5):475-92. doi: 10.1085/jgp.34.5.475.
3
An icesolvent method of drying frozen tissue for plant cytology.一种用于植物细胞学的冷冻组织干燥的冰溶剂法。
Stain Technol. 1955 May;30(3):123-31. doi: 10.3109/10520295509113755.
4
A new method for the isolation of deoxyribonucleic acids; evidence on the nature of bonds between deoxyribonucleic acid and protein.一种分离脱氧核糖核酸的新方法；关于脱氧核糖核酸与蛋白质之间键的性质的证据。
Biochem J. 1957 Jul;66(3):495-504. doi: 10.1042/bj0660495.
5
Studies on nucleoproteins. V. Interactions.核蛋白研究。V. 相互作用
J Biol Chem. 1957 May;226(1):157-64.
6
Microspectrophotometric study of the binding of the anionic dye, naphthol yellow S, by tissue sections and by purified proteins.阴离子染料萘酚黄S与组织切片及纯化蛋白质结合的显微分光光度研究。
Lab Invest. 1955 Sep-Oct;4(5):324-51.

RNA和DNA都可能与同一种蛋白质形成复合物的细胞学证据。

Cytological evidence that both RNA and DNA may form a complex with the same protein.

作者信息

LITTAU V C

出版信息

J Biophys Biochem Cytol. 1959 Mar 25;5(2):231-4. doi: 10.1083/jcb.5.2.231.

DOI:10.1083/jcb.5.2.231
PMID:13654441
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2224651/
Abstract

Deoxyribonucleic acid can be added back to protein sites from which the original nucleic acid, ribo- or deoxyribo-, is removed. If sections of frozen-substituted ovarian follicle cells of a leafhopper are first extracted by hot trichloracetic acid to remove nucleic acids and then immersed in a solution of a commerical preparation of deoxyribonucleic acid, the nucleic acid becomes attached to nuclear and cytoplasmic sites and can be rendered visible by the Feulgen reaction. The addition occurs in certain other tissues as well. The results are discussed in relation to biochemical and other cytochemical investigations of the nucleoprotein complex.

摘要

脱氧核糖核酸可以重新添加到蛋白质位点上，而这些位点上原来的核酸（核糖核酸或脱氧核糖核酸）已被去除。如果先将叶蝉冷冻替代的卵巢卵泡细胞切片用热三氯乙酸提取以去除核酸，然后浸入商业制备的脱氧核糖核酸溶液中，核酸就会附着在细胞核和细胞质位点上，并可通过福尔根反应使其可见。这种添加也发生在某些其他组织中。结合核蛋白复合物的生化和其他细胞化学研究对结果进行了讨论。