Ennaceur A, Meliani K
Laboratoire de Psychophysiologie, Université Paris VII, France.
Psychopharmacology (Berl). 1992;109(3):321-30. doi: 10.1007/BF02245880.
The effects of physostigmine and scopolamine were evaluated on working memory of rats in object recognition and radial-maze tests. Three doses of physostigmine hemi-sulfate (Phys: 0.05, 0.10 and 0.20 mg/kg), five doses of scopolamine hydrobromide (Scop: 0.125, 0.25, 0.5, 1.0 and 2.0 mg/kg), and one dose of scopolamine methylbromide (Mscop: 2.0 mg/kg) were used. In object recognition test, rats were submitted to three or four intertrial delay conditions (1-min, 15-min and either 60-min or 24-h). The higher doses of Scop (1.0 and 2.0 mg/kg) in 1-min and 15-min delay and of Phys (0.20 mg/kg) in 1-min delay impaired discrimination between new and familiar objects. Mscop impaired discrimination between objects in 60-min but not in 1-min and 15-min delay. This effect may be state dependent. Radial-maze learning was impaired by the lower doses of scopolamine (0.25 and 0.50 mg/kg) which had no effect in object recognition test. These results show that in our conditions, object recognition is less sensitive than radial-maze test to cholinergic drugs.
在物体识别和放射状迷宫试验中评估了毒扁豆碱和东莨菪碱对大鼠工作记忆的影响。使用了三种剂量的半硫酸毒扁豆碱（毒扁豆碱：0.05、0.10和0.20毫克/千克）、五种剂量的氢溴酸东莨菪碱（东莨菪碱：0.125、0.25、0.5、1.0和2.0毫克/千克）以及一种剂量的甲基溴化东莨菪碱（甲基东莨菪碱：2.0毫克/千克）。在物体识别试验中，大鼠被置于三种或四种试验间隔条件下（1分钟、15分钟以及60分钟或24小时）。在1分钟和15分钟延迟条件下，较高剂量的东莨菪碱（1.0和2.0毫克/千克）以及在1分钟延迟条件下较高剂量的毒扁豆碱（0.20毫克/千克）损害了对新物体和熟悉物体的辨别能力。甲基东莨菪碱在60分钟延迟条件下损害了物体辨别能力，但在1分钟和15分钟延迟条件下没有。这种效应可能与状态有关。较低剂量的东莨菪碱（0.25和0.50毫克/千克）损害了放射状迷宫学习能力，而这些剂量在物体识别试验中没有影响。这些结果表明，在我们的实验条件下，物体识别对胆碱能药物的敏感性低于放射状迷宫试验。
