Kalmanti M, Kanavaros P, Sakalidou A, Tzardi M, Datseris G, Bolonaki E, Kalmantis T, Kazlaris E, Delides G
Dept. of Paediatric Haematology-Oncology and Pathology, University Hospital Heraklion, Greece.
Acta Morphol Hung. 1992;40(1-4):215-21.
Paraffin sections from 21 cases of Hodgkin's disease (HD) and 28 cases (26 high-grade and 2 low-grade) of non-Hodgkin's lymphomas (NHL) occurring in childhood were examined for the presence of proliferating cell nuclear antigen using an anti-PCNA antibody. All cases of HD and NHL showed PCNA reactivity. In HD 50.9% (mean value) of Hodgkin and Reed-Sternberg (HRS) cells were PCNA positive and judged to be proliferating. PCNA reactivity was also found in a varying number of cells of the background population in HD (mean value = 11.7%). In NHL 61.2% (mean value) of cells were PCNA positive. In the 26 high grade tumours 63.6% (mean value) of cells were PCNA positive while only 32% (mean value) of cells were PCNA positive in the 2 low-grade tumours. Our results show that the proliferation rate of tumour cells in high-grade NHL is higher than those of tumour cells in low-grade NHL and HRS cells in HD. Moreover, we found a considerable variation of proliferation rate among individual cases of HD (range 31%-68%) of NHL (range 31%-78%). This suggests that PCNA assessment can help in the individual approach of the proliferation rate of each tumour, and, in conjunction with other parameters of the cell proliferation, could be useful for the understanding of the biological behavior of childhood lymphomas.
使用抗增殖细胞核抗原（PCNA）抗体，对21例霍奇金淋巴瘤（HD）以及28例儿童非霍奇金淋巴瘤（NHL，其中26例为高级别，2例为低级别）的石蜡切片进行检测，以确定是否存在增殖细胞核抗原。所有HD和NHL病例均显示PCNA反应性。在HD中，50.9%（平均值）的霍奇金和里德-斯腾伯格（HRS）细胞PCNA呈阳性，并被判定为处于增殖状态。在HD背景细胞群中也发现了不同数量的PCNA反应性细胞（平均值 = 11.7%）。在NHL中，61.2%（平均值）的细胞PCNA呈阳性。在26例高级别肿瘤中，63.6%（平均值）的细胞PCNA呈阳性，而在2例低级别肿瘤中，只有32%（平均值）的细胞PCNA呈阳性。我们的结果表明，高级别NHL中肿瘤细胞的增殖率高于低级别NHL和HD中的HRS细胞。此外，我们发现HD（范围31%-68%）和NHL（范围31%-78%）的各个病例之间增殖率存在相当大的差异。这表明PCNA评估有助于针对每个肿瘤的增殖率采取个体化方法，并且与细胞增殖的其他参数相结合，可能有助于了解儿童淋巴瘤的生物学行为。
