Kamel O W, Warnke R A, Banks P M
Department of Pathology, Stanford University Medical Center, CA 94305.
Semin Diagn Pathol. 1992 Nov;9(4):311-4.
Proliferating cell nuclear antigen (PCNA/Cyclin) is a 36-kD protein that is present in cycling cells but not in resting cells, and therefore represents a marker of tumor proliferation. Application of anti-PCNA/Cyclin monoclonal antibodies has shown that this protein is localized to the nucleus of cycling cells, with the exception of cells in mitosis, which demonstrate faint cytoplasmic reactivity. Recently, Benjamin and Gown found that Reed-Sternberg cells and variants show nuclear and cytoplasmic staining with anti-PCNA/Cyclin antibody 19A2, and suggested that this feature may be useful in distinguishing Hodgkin's disease from other tumors. This report describes the reactivity of 42 workshop cases that were stained with anti-PCNA/Cyclin antibodies 19A2 and/or PC10. Thirty-three (79%) of the 42 cases showed adequate reactivity to allow for interpretation of staining localization. In the group of reactive cases, 26 (79%) showed nuclear and cytoplasmic staining. The localization of PCNA/Cyclin was compared with the consensus diagnosis in each case. Eighty percent of cases classified as Hodgkin's disease, 67% of cases classified as non-Hodgkin's lymphoma, and 100% of unresolved cases showed both nuclear and cytoplasmic staining. The incidence of cytoplasmic PCNA/Cyclin was not different between Hodgkin's disease and non-Hodgkin's lymphoma in this study.
增殖细胞核抗原（PCNA/细胞周期蛋白）是一种36kD的蛋白质，存在于处于增殖周期的细胞中，而不存在于静止细胞中，因此是肿瘤增殖的一个标志物。应用抗PCNA/细胞周期蛋白单克隆抗体已表明，这种蛋白质定位于增殖周期细胞的细胞核，但有丝分裂期的细胞除外，这些细胞显示出微弱的细胞质反应性。最近，本杰明和高恩发现，里德-斯腾伯格细胞及其变异型在用抗PCNA/细胞周期蛋白抗体19A2染色时显示细胞核和细胞质着色，并提示这一特征可能有助于将霍奇金病与其他肿瘤区分开来。本报告描述了用抗PCNA/细胞周期蛋白抗体19A2和/或PC10染色的42例研讨病例的反应性。42例中的33例（79%）显示出足够的反应性，以便对染色定位进行解读。在反应性病例组中，26例（79%）显示细胞核和细胞质染色。将PCNA/细胞周期蛋白的定位与每例的一致诊断进行了比较。分类为霍奇金病的病例中有80%、分类为非霍奇金淋巴瘤的病例中有67%以及未确诊病例中有100%均显示细胞核和细胞质染色。在本研究中，霍奇金病和非霍奇金淋巴瘤之间细胞质PCNA/细胞周期蛋白的发生率没有差异。
