Tesco G, Latorraca S, Piersanti P, Piacentini S, Amaducci L, Sorbi S
Department of Neurological and Psychiatric Sciences, University of Florence, Italy.
Mech Ageing Dev. 1992 Nov;66(2):117-20. doi: 10.1016/0047-6374(92)90129-2.
We have studied the response to toxic oxygen metabolites of fibroblasts derived from skin biopsies of 5 patients with familial (FAD) and 4 with sporadic (AD) Alzheimer's disease compared with those derived from 4 normal controls. Fibroblasts were damaged by the generation of oxygen metabolites during the enzymatic oxidation of acetaldehyde by 50 munits of xanthine-oxidase (Xo). To quantify cell damage we measured lactate dehydrogenase (LDH) activity in the culture medium and cell viability in fibroblast cultures. We found a significant increase in LDH activity in the FAD vs. controls and also in the AD vs. controls.
我们研究了来自5例家族性（FAD）和4例散发性（AD）阿尔茨海默病患者皮肤活检组织的成纤维细胞对有毒氧代谢产物的反应，并与来自4例正常对照者的成纤维细胞进行了比较。在50单位黄嘌呤氧化酶（Xo）将乙醛酶促氧化过程中，氧代谢产物的生成会损伤成纤维细胞。为了量化细胞损伤，我们测定了培养基中的乳酸脱氢酶（LDH）活性以及成纤维细胞培养物中的细胞活力。我们发现，与对照组相比，FAD组和成纤维细胞的LDH活性显著增加，AD组也如此。
