Spector J I, Zaroulis C G, Pivacek L E, Emerson C P, Valeri C R
Am J Physiol. 1977 Jan;232(1):H79-84. doi: 10.1152/ajpheart.1977.232.1.H79.
Baboons were bled one-third their red cell mass and were given homologous transfusions of red blood cells to restore the red cell volume. One group of baboons received red blood cells with a normal 2,3-diphosphoglycerate 2,3-DPG) level and normal affinity for oxygen, and in this group the 2,3-DPG level after transfusion was normal. The other group received red blood cells with a 160% of normal 2,3-DPG level and decreased affinity for oxygen, and in this group the 2,3-DPG level after transfusion was 125% of normal. In both groups of baboons, the inspired oxygen concentration was lowered and arterial PO2 tension was maintained at 55-60 mmHg for 2 h after transfusion. During the hypoxic state, systemic oxygen extraction was similar in the two groups, whereas oxygen saturation was lower in the high 2,3-DPG group than in the control animals. Cardiac output was significantly reduced 30 min after the arterial PO2 was restored to normal. These data indicate that red blood cells with decreased affinity for oxygen maintained satisfactory oxygen delivery to tissue during hypoxia.
对狒狒进行放血，使其红细胞量减少三分之一，然后给予同源红细胞输血以恢复红细胞量。一组狒狒接受2,3 -二磷酸甘油酸（2,3-DPG）水平正常且对氧亲和力正常的红细胞，该组输血后2,3-DPG水平正常。另一组接受2,3-DPG水平为正常160%且对氧亲和力降低的红细胞，该组输血后2,3-DPG水平为正常的125%。在两组狒狒中，输血后降低吸入氧浓度并将动脉血氧分压维持在55 - 60 mmHg达2小时。在低氧状态下，两组的全身氧摄取相似，而高2,3-DPG组的氧饱和度低于对照动物。在动脉血氧分压恢复正常30分钟后，心输出量显著降低。这些数据表明，对氧亲和力降低的红细胞在低氧期间能维持向组织的满意氧输送。
