Bi Y, Hong W B
Department of Scientific Instruments, Zhejiang University, China.
Biotechnol Prog. 1990 Jan-Feb;6(1):62-6. doi: 10.1021/bp00001a010.
Several liquid membrane microelectrodes sensitive to bile acids (two barrel, tip diameter about 0.5 micron) are described. The results of different liquid ion exchangers such as Aliquat 336/decanol, trioctylmethylammonium/decanol, hexadecyltrimethylammonium/decanol, benzyldimethylhexadecylammonium/decanol, hexadecyltributylammonium/5% hexachlorobenzene + 0.5% bromoacetanilide in o-dichlorobenzene are compared with each other, and the better one among them is the mixture of benzyldimethylhexadecylammonium cholate/decanol with hexadecyltributylammonium taurocholate/5% hexachlorobenzene + 0.5% bromoacetanilide in o-dichlorobenzene because of its quicker response time and low drift. The calibration curves, slopes, test limits, selective coefficients, drifts, and response times of the various bile-acids-sensitive microelectrodes in different calibration solutions were demonstrated and compared with each other.
描述了几种对胆汁酸敏感的液膜微电极（双管，尖端直径约0.5微米）。比较了不同的液体离子交换剂，如Aliquat 336/癸醇、三辛基甲基铵/癸醇、十六烷基三甲基铵/癸醇、苄基二甲基十六烷基铵/癸醇、十六烷基三丁基铵/邻二氯苯中的5%六氯苯+0.5%溴乙酰苯胺，其中较好的是胆酸苄基二甲基十六烷基铵/癸醇与牛磺胆酸十六烷基三丁基铵/邻二氯苯中的5%六氯苯+0.5%溴乙酰苯胺的混合物，因为其响应时间更快且漂移低。展示并比较了各种胆汁酸敏感微电极在不同校准溶液中的校准曲线、斜率、测试极限、选择性系数、漂移和响应时间。
