Kunze L J, Lindenbaum S
Department of Pharmaceutical Chemistry, University of Kansas, Lawrence 66045-2504.
J Pharm Biomed Anal. 1989;7(8):947-54. doi: 10.1016/0731-7085(89)80019-6.
The development of a prototype ion-selective microelectrode for bile salts is described. The microelectrodes demonstrated Nernstian response (59 +/- 10 mV dec-1) with sodium deoxycholate in Tris (0.2 M, pH 9.0, 15-30 degrees C), HEPES (0.13 M, pH 7.5), and bicarbonate buffers (0.1 M, pH 7.5) (25 +/- 0.1 degrees C) were stable for several days, and the responses were highly reproducible. The microelectrodes were selective for bile salts over the physiologically important inorganic anions, bicarbonate and chloride. The response to sodium cholate (42 mV dec-1) was consistently lower than the ideal response (59 mV dec-1). This ion-selective microelectrode may show promise as a useful tool for the determination of intracellular bile salt activity.
本文描述了一种用于胆汁盐的离子选择性微电极原型的开发。该微电极在Tris（0.2M，pH 9.0，15 - 30℃）、HEPES（0.13M，pH 7.5）和碳酸氢盐缓冲液（0.1M，pH 7.5）（25±0.1℃）中对脱氧胆酸钠表现出能斯特响应（59±10 mV dec-1），可稳定数天，且响应具有高度可重复性。该微电极对胆汁盐的选择性高于生理上重要的无机阴离子——碳酸氢根和氯离子。对胆酸钠的响应（42 mV dec-1）始终低于理想响应（59 mV dec-1）。这种离子选择性微电极有望成为测定细胞内胆汁盐活性的有用工具。
