Horvath M, Ditzelmüller G, Loidl M, Streichsbier F
Abteilung für Mikrobielle Okologie und Methodik am Institut für Biochemische Technologie und Mikrobiologie, Technische Universität Wien, Vienna, Austria.
Appl Microbiol Biotechnol. 1990 May;33(2):213-6. doi: 10.1007/BF00176527.
The 2-(2,4-dichlorphenoxy)propionic acid (2,4-DP)-degrading bacterial strain MH was isolated after numerous subcultivations of a mixed culture obtained by soil-column enrichment and finally identified as Flavobacterium sp. Growth of this strain was supported by 2,4-DP (maximum specific growth rate 0.2 h-1) as well as by 2,4-dichlorophenoxyacetic acid (2,4-D), 4-(2,4-dichlorophenoxy)butyric acid (2,4-DB), and 2-(4-chloro-2-methylphenoxy)propionic acid (MCPP) as sole sources of carbon and energy under aerobic conditions. 2,4-DP-Grown cells (10(8] of strain MH degraded 2,4-dichlorophenoxyalkanoic acids, 2,4-dichlorophenol (2,4-DCP), and 4-chlorophenol at rates in the range of 30 nmol/h. Preliminary investigations indicate that cleavage of 2,4-DP results in 2,4-DCP, which is further mineralized via ortho-hydroxylation and ortho-cleavage of the resulting 3,5-dichlorocatechol.
通过土壤柱富集获得的混合培养物经多次传代培养后，分离出了能降解2-(2,4-二氯苯氧基)丙酸(2,4-DP)的细菌菌株MH，最终鉴定为黄杆菌属。该菌株在有氧条件下，以2,4-DP（最大比生长速率0.2 h-1）以及2,4-二氯苯氧基乙酸(2,4-D)、4-(2,4-二氯苯氧基)丁酸(2,4-DB)和2-(4-氯-2-甲基苯氧基)丙酸(MCPP)作为唯一碳源和能源时能够生长。以2,4-DP培养的细胞（菌株MH的10^8个）以30 nmol/h的速率降解2,4-二氯苯氧基链烷酸、2,4-二氯苯酚(2,4-DCP)和4-氯苯酚。初步研究表明，2,4-DP的裂解产生2,4-DCP，其通过所得3,5-二氯邻苯二酚的邻位羟基化和邻位裂解进一步矿化。
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