Oh K H, Tuovinen O H
Department of Microbiology, Ohio State University, Columbus.
FEMS Microbiol Lett. 1991 Apr 15;63(2-3):141-6. doi: 10.1016/0378-1097(91)90076-m.
The concurrent bacterial degradation of 2-(2-methyl-4-chlorophenoxy)propionic acid and 2,4-dichlorophenoxyacetic acid was studied using a stirred tank reactor and a bacterial culture which had been originally derived by enrichment with MCPP. High pressure liquid chromatographic methodology was used to measure both herbicides and it also resolved the corresponding phenols as intermediates, i.e., 2-methyl-4-chlorophenol and 2,4-dichlorophenol. Gas chromatography-mass spectrometry was used to verify the intermediates. UV scans of spent cultures showed that the wave-length of maximum absorption shifted from 282 nm to 280 nm toward the end of incubation, but the characteristic peaks of maximum absorption of these compounds could not be used resolved because of the overlap.
使用搅拌釜式反应器和最初通过用2-(2-甲基-4-氯苯氧基)丙酸（MCPP）富集得到的细菌培养物，研究了2-(2-甲基-4-氯苯氧基)丙酸和2,4-二氯苯氧基乙酸的同步细菌降解。采用高压液相色谱法测定两种除草剂，并将相应的酚类中间体（即2-甲基-4-氯苯酚和2,4-二氯苯酚）分离出来。利用气相色谱-质谱联用仪对中间体进行验证。对用过的培养物进行紫外扫描显示，在培养末期，最大吸收波长从282 nm移至280 nm，但由于这些化合物最大吸收的特征峰重叠，无法将其分离。
