Guzy S, Saidel G M, Lotan N
Department of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa.
Biotechnol Prog. 1990 Mar-Apr;6(2):98-103. doi: 10.1021/bp00002a002.
In a previous report, we presented a new analytical model describing the performance of a packed-bed catalytic unit, where the reaction between two cosubstrates is catalyzed by an enzyme immobilized on a porous carrier. The model explicitly takes into account the changes in concentrations of both cosubstrates along the reactor, as well as the hydrodynamic regimen (i.e., back-mixing) prevailing in the packed bed. In the present report, and on the basis of the procedures developed, we present a detailed analysis of the performance of the reactor. With numerical simulations, the effects of internal diffusion limitations, the depth of the pores, the substrates' concentration in the feed, and kinetic parameters are evaluated. Particular attention is also given here to the back-mixing effects prevailing in the reactor. An experimental procedure for assessing their extent is described.
在之前的一份报告中，我们提出了一个新的分析模型，该模型描述了填充床催化装置的性能，其中两种共底物之间的反应由固定在多孔载体上的酶催化。该模型明确考虑了两种共底物在反应器内浓度的变化，以及填充床中普遍存在的流体动力学状态（即返混）。在本报告中，基于所开发的程序，我们对反应器的性能进行了详细分析。通过数值模拟，评估了内部扩散限制、孔深度、进料中底物浓度和动力学参数的影响。这里还特别关注了反应器中普遍存在的返混效应。描述了一种评估其程度的实验程序。
