Sakoda A, Nigam S C, Wang H Y
Department of Chemical Engineering, University of Michigan, Ann Arbor 48109.
Enzyme Microb Technol. 1990 May;12(5):349-54. doi: 10.1016/0141-0229(90)90163-k.
A new approach for isolating and recovering biological macromolecules using membrane-encapsulated soluble ligand conjugates was investigated. Membrane-encapsulated solid adsorbents have been successfully developed and employed in our laboratory to isolate and purify proteins and enzymes directly from culture broths. This new concept also makes it possible to use soluble ligand conjugates instead of solid adsorbents inside membrane capsules. In this work, model membrane-encapsulated soluble and insoluble ligands comprising Blue Dextran and Blue Sepharose entrapped within calcium alginate membranes were studied to compare adsorption characteristics such as capacities and rates. Experimental results suggest that membrane-encapsulated soluble ligands may be expected to result in higher overall adsorption capacity compared to membrane-encapsulated solid adsorbents with comparable adsorption rates.
研究了一种使用膜包封的可溶性配体缀合物分离和回收生物大分子的新方法。膜包封的固体吸附剂已在我们实验室成功开发并用于直接从培养液中分离和纯化蛋白质及酶。这一新概念还使得在膜胶囊内使用可溶性配体缀合物而非固体吸附剂成为可能。在这项工作中，研究了包含包埋在海藻酸钙膜内的蓝色葡聚糖和蓝色琼脂糖的膜包封可溶性和不溶性配体模型，以比较吸附容量和速率等吸附特性。实验结果表明，与具有可比吸附速率的膜包封固体吸附剂相比，膜包封可溶性配体有望产生更高的总吸附容量。
