Boisseau S, Tamponnet C, Poujeol C, Lièvremont M, Simonneau M
Laboratoire de Neurobiologie Cellulaire et Moléculaire CNRS, Gif sur Yvette, France.
Biomater Artif Cells Immobilization Biotechnol. 1993;21(3):421-6. doi: 10.3109/10731199309117381.
We developed improved immobilization conditions which permitted (i) to immobilize neuroblastoma cells (N18) in calcium-alginate gel beads, (ii) to test the function of ionic channels using patch-clamp electrophysiological techniques and (iii) to quantitatively analyze ligand interactions with voltage-dependent sodium channels in neurons inside the beads. These results qualify this immobilization technique for the isolation and/or purification of ligands specific for neuronal cells.
我们开发了改良的固定条件，该条件允许：（i）将神经母细胞瘤细胞（N18）固定在海藻酸钙凝胶珠中；（ii）使用膜片钳电生理技术测试离子通道的功能；（iii）定量分析配体与珠内神经元中电压依赖性钠通道的相互作用。这些结果证明了这种固定技术可用于分离和/或纯化对神经元细胞具有特异性的配体。
