HARDING C V, FELDHERR C
J Gen Physiol. 1959 Jul 20;42(6):1155-65. doi: 10.1085/jgp.42.6.1155.
The osmotic properties of nuclei in intact cells were studied by injecting solutions into the cytoplasm of amphibian oocytes. Subsequent changes in nuclear volume were recorded photographically. The injection of solutions containing polyvinylpyrrolidone or bovine serum albumin caused changes in nuclear volume which were related to the colloid osmotic pressure of the solution injected. The concentration in which no significant nuclear volume change occurred (the isotonic range) was 1.0 to 1.5 per cent polyvinylpyrrolidone (2.0 to 3.75 x 10(-4)M). 2 per cent bovine serum albumin had no significant effect on nuclear volume, whereas 4 per cent caused a significant decrease. The significance of these findings is discussed in terms of the permeability characteristics of the nuclear membrane.
通过将溶液注入两栖类卵母细胞的细胞质中，研究了完整细胞中细胞核的渗透特性。随后通过摄影记录细胞核体积的变化。注入含有聚乙烯吡咯烷酮或牛血清白蛋白的溶液会引起细胞核体积的变化，这种变化与注入溶液的胶体渗透压有关。未发生明显细胞核体积变化的浓度范围（等渗范围）为1.0%至1.5%的聚乙烯吡咯烷酮（2.0至3.75×10⁻⁴M）。2%的牛血清白蛋白对细胞核体积无明显影响，而4%则会导致显著减小。根据核膜的通透性特征对这些发现的意义进行了讨论。
