Godbole S S, Kubal B S, D'Souza S F
Food Technology and Enzyme Engineering Division, Bhabha Atomic Research Centre, Bombay, India.
Enzyme Microb Technol. 1990 Mar;12(3):214-7. doi: 10.1016/0141-0229(90)90041-n.
Yeast invertase was immobilized on polyethyleneimine-coated cotton thread by adsorption followed by crosslinking with glutaraldehyde. The thread-bound invertase was used as an easily retrievable system for the hydrolysis of 80% w/v commercial sucrose syrups. The immobilized enzyme was stable for over 90 days to a temperature of 50 degrees C, only when stored in 80% sucrose solution. Above this temperature, inactivation of enzyme was observed. The cotton threads were used in a batch reactor for hydrolysis of sucrose in about 30 batches carried out over a period of 50 days without loss in activity. The threads could also be used in a packed bed reactor (1.51) for 97% hydrolysis of 80% sucrose syrups at 50 degrees C at a rate of about 360 kg per month for a period of 3 months.
通过吸附将酵母转化酶固定在聚乙烯亚胺包被的棉线上，然后用戊二醛交联。固定在棉线上的转化酶用作一种易于回收的系统，用于水解80%（w/v）的商业蔗糖糖浆。固定化酶在50℃温度下，仅当储存在80%蔗糖溶液中时，稳定超过90天。高于此温度，观察到酶失活。棉线在间歇反应器中用于蔗糖水解，在50天内进行了约30批次反应，活性没有损失。这些棉线也可用于填充床反应器（1.5升），在50℃下以约每月360千克的速率对80%蔗糖糖浆进行97%的水解，持续3个月。
