Kinetics of hydrolysis of sucrose catalyzed by invertase immobilized on egg shells and on zeolites.

A simple, inexpensive and reliable method for immobilizing yeast invertase on egg shells and on zeolites was developed. The activity of the immobilized preparations when compared with that of native enzyme at varying pH, temperature and substrate concentrations, showed improved stability and sigmoidal kinetic behaviour. The immobilized enzyme could be easily removed from the reaction mixture at any specified time.