Weatherley L R, Campbell I, Kirton D, Slaughter J C
Department of Chemical and Process Engineering, Heriot Watt University, Currie, Edinburgh, UK.
J Chem Technol Biotechnol. 1990;48(4):427-38. doi: 10.1002/jctb.280480405.
The application of electrically enhanced liquid-liquid extraction techniques to the recovery and purification of penicillin G from aqueous solutions and from untreated mycelial culture broth is described. Experiments at laboratory scale have shown that extraction rates into dichloromethane at pH 4.0 may be increased by factors of up to five-fold by electrostatically spraying penicillin G solutions into a continuum of solvent held in electrical tension. The experimental studies revealed that in small spray column contactors, electrostatic spray conditions, once initiated at nozzle voltages in excess of 10 kV, could be sustained at nozzle voltages as little as 4 kV. The results demonstrate the potential of this technique as an alternative to mechanically augmented liquid-liquid contact for the intensification of whole broth extraction processes.
描述了电增强液-液萃取技术在从水溶液和未处理的菌丝体培养液中回收和纯化青霉素G方面的应用。实验室规模的实验表明，通过将青霉素G溶液静电喷雾到处于电张力的连续溶剂中，在pH 4.0时进入二氯甲烷的萃取率可提高多达五倍。实验研究表明，在小型喷雾柱接触器中，静电喷雾条件一旦在超过10 kV的喷嘴电压下启动，在低至4 kV的喷嘴电压下也能维持。结果证明了该技术作为强化全培养液萃取过程的机械增强液-液接触替代方法的潜力。
