反应介质对过氧化物酶催化对甲酚氧化产物分布的影响。

Influence of the reaction medium on the product distribution of peroxidase-catalysed oxidation of p-cresol.

作者信息

Pietikäinen P, Adlercreutz P

机构信息

Department of Biotechnology, University of Lund, Sweden.

出版信息

Appl Microbiol Biotechnol. 1990 Jul;33(4):455-8. doi: 10.1007/BF00176665.

DOI:10.1007/BF00176665

PMID:1366748

Abstract

p-Cresol was oxidized by hydrogen peroxide in a reaction catalysed by horseradish peroxidase and the low molecular weight products were investigated. In aqueous media Pummerer's ketone (I) was the dominating product but in organic media the product distribution was quite different; 2,2'-dihydroxy-5,5'-dimethyldiphenyl (II) was the main low molecular weight product. Similar product distributions were obtained with peroxidase adsorbed on a solid support and suspended in toluene and with peroxidase solubilized in a microemulsion containing the same solvent. The best selectivity for the formation of (II) was obtained when the enzyme was adsorbed on Celite and suspended in water-saturated chloroform with 0.5% (v/v) extra water added. The yield of low molecular weight products in this case was 28%; of this fraction, 95% was (II).

摘要

对甲酚在辣根过氧化物酶催化的反应中被过氧化氢氧化，并对低分子量产物进行了研究。在水介质中，普默勒酮（I）是主要产物，但在有机介质中，产物分布有很大不同；2,2'-二羟基-5,5'-二甲基二苯基（II）是主要的低分子量产物。将过氧化物酶吸附在固体载体上并悬浮于甲苯中，以及将过氧化物酶溶解在含有相同溶剂的微乳液中，都得到了类似的产物分布。当酶吸附在硅藻土上并悬浮于添加了0.5%（v/v）额外水的水饱和氯仿中时，对（II）的形成具有最佳选择性。在这种情况下，低分子量产物的产率为28%；在该部分中，95%是（II）。