Kim K S, Pai H S, Lee S Y, Ryu D D
Department of Chemical Engineering, University of California, Davis 95616.
Enzyme Microb Technol. 1990 Aug;12(8):564-70. doi: 10.1016/0141-0229(90)90128-d.
The effect of treatment with various intercalating dyes on the ability to produce antibiotics in Micromonospora rosaria and Micromonospora purpurea was studied. Treatment with acriflavine resulted in a high frequency loss of antibiotic productivity in both species. In M. rosaria, the loss of antibiotic-producing ability appeared to be strain-dependent. In M. purpurea, up to 90% of colonies were found to have lost gentamicin-producing ability when protoplasts were used in the test. These antibiotic-nonproducing strains were further studied. The following observations were made: (1) Unlike the producing ability, the resistance to the antibiotics is a very stable character in both species. (2) Protoplast fusion analysis indicates that rosamicin-nonproducing characteristics of MR 217-AF2 and MR 217-AF3 strains induced by the acriflavine treatment is due to chromosomal mutation or rearrangement but not to loss of a plasmid. (3) Gentamicin-nonproducing strains of M. purpurea responded differently to the supplementation of streptamine or DOS in the culture medium. When supplemented with streptamine or DOS, some of these strains regained the ability to produce antibiotic, showing that the biosynthesis of intermediate was affected in these strains.
研究了用各种嵌入染料处理对玫瑰小单孢菌和紫色小单孢菌产生抗生素能力的影响。用吖啶黄素处理导致这两个物种的抗生素生产力高频丧失。在玫瑰小单孢菌中，抗生素产生能力的丧失似乎与菌株有关。在紫色小单孢菌中，当在试验中使用原生质体时，发现高达90%的菌落失去了产生庆大霉素的能力。对这些不产生抗生素的菌株进行了进一步研究。得出以下观察结果：(1) 与产生能力不同，这两个物种对抗生素的抗性是非常稳定的特征。(2) 原生质体融合分析表明，吖啶黄素处理诱导的MR 217-AF2和MR 217-AF3菌株不产生玫瑰霉素的特性是由于染色体突变或重排，而不是由于质粒丢失。(3) 紫色小单孢菌的不产生庆大霉素的菌株对培养基中添加链霉胺或DOS的反应不同。当添加链霉胺或DOS时，其中一些菌株恢复了产生抗生素的能力，表明这些菌株中中间体的生物合成受到了影响。
