罗沙霉素产生菌 Micromonospora rosaria 中细胞色素 P450 酶 RosC 和 RosD 的功能在罗沙霉素大环内酯抗生素生物合成中的作用。
Function of cytochrome P450 enzymes RosC and RosD in the biosynthesis of rosamicin macrolide antibiotic produced by Micromonospora rosaria.
机构信息
Faculty of Pharmaceutical Sciences, Toho University, Funabashi, Chiba, Japan.
出版信息
Antimicrob Agents Chemother. 2013 Mar;57(3):1529-31. doi: 10.1128/AAC.02092-12. Epub 2012 Dec 28.
Abstract
The cytochrome P450 enzyme-encoding genes rosC and rosD were cloned from the rosamicin biosynthetic gene cluster of Micromonospora rosaria IFO13697. The functions of RosC and RosD were demonstrated by gene disruption and complementation with M. rosaria and bioconversion of rosamicin biosynthetic intermediates with Escherichia coli expressing RosC and RosD. It is proposed that M. rosaria IFO13697 has two pathway branches that lead from the first desosaminyl rosamicin intermediate, 20-deoxo-20-dihydro-12,13-deepoxyrosamicin, to rosamicin.
摘要
从玫瑰链霉菌（Micromonospora rosaria）IFO13697 的罗沙米星生物合成基因簇中克隆了细胞色素 P450 酶编码基因 rosC 和 rosD。通过基因敲除和 M. rosaria 的基因互补以及表达 RosC 和 RosD 的大肠杆菌对罗沙米星生物合成中间体的生物转化，证明了 RosC 和 RosD 的功能。提出 M. rosaria IFO13697 有两条途径分支，从第一个去糖基罗沙米星中间体 20-去氧-20-二氢-12,13-去氧罗沙米星到罗沙米星。
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