Gailliot F P, Gleason C, Wilson J J, Zwarick J
Merck & Co., Inc., Rahway, New Jersey 07065.
Biotechnol Prog. 1990 Sep-Oct;6(5):370-5. doi: 10.1021/bp00005a009.
Fluidized bed adsorption using a high-density synthetic resin has proven to be an invaluable technique for separating novel compounds from unfiltered fermentation broths during the very early stages of fermentation development, where product concentrations are typically in the parts per million range. Previous initial downstream processing strategies consisted of cell separation from whole broth or direct extraction with water-immiscible solvents, both of which resulted in lengthy time cycles, conflicts with existing operations requiring the use of high-cost centrifugal separators, and environmental/solvent recovery concerns. Laboratory and subsequent pilot plant process development work along with concomitant improvements in yield, quality, and time cycles are presented for one of several fluidized bed processes piloted in Merck's Natural Product Isolation facility.
在发酵开发的早期阶段，当产物浓度通常处于百万分之一范围内时，使用高密度合成树脂的流化床吸附已被证明是一种从未过滤的发酵液中分离新化合物的宝贵技术。以前的初始下游加工策略包括从全发酵液中分离细胞或用与水不混溶的溶剂直接萃取，这两种方法都导致时间周期长、与需要使用高成本离心分离器的现有操作相冲突以及存在环境/溶剂回收问题。本文介绍了在默克天然产物分离设施中进行试点的几种流化床工艺之一的实验室及后续中试工厂工艺开发工作，以及随之而来的产量、质量和时间周期方面的改进。
