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CS16GC和IRC747树脂对发酵液中γ-氨基丁酸的扩张床吸附

Expanded Bed Adsorption of -Aminobutyric Acid from broth by CS16GC and IRC747 Resins.

作者信息

Pathapati Trinath, Rütze Dennis N, den Boer Piet, de Wit Pieter, Zaalberg Menne

机构信息

Xendo B.V. Bio Science Park, Schipholweg 73-75 2316 ZL Leiden The Netherlands.

出版信息

Chem Eng Technol. 2018 Dec;41(12):2427-2434. doi: 10.1002/ceat.201800295. Epub 2018 Oct 30.

DOI:10.1002/ceat.201800295
PMID:31007410
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6472577/
Abstract

Expanded-bed adsorption (EBA) is an efficient downstream technology that enhances the techno-economic potential of bio-based industries. However, application of EBA for bulk biochemicals requires the use of industrial resins. Therefore, two cation exchangers, namely, gel-type CS16GC and porous IRC747, were tested to purify -aminobutyric acid (GABA) from unclarified fermentation broth. Experiments compared the impact of gel-type and macroporous resin properties on the EBA process performance. As an outcome, the gel-type resin exhibited higher GABA binding capacity of compared to that of macroporous resin. This was due to improved hydrodynamics and uniform flow distribution in the case of gel-type resin. Further, CS16GC effectively removed ≥ 99 % of impurities and achieved ≥ 97 % GABA yield.

摘要

扩张床吸附（EBA）是一种高效的下游技术，可提高生物基产业的技术经济潜力。然而，将EBA应用于大宗生化产品需要使用工业树脂。因此，测试了两种阳离子交换剂，即凝胶型CS16GC和多孔型IRC747，以从未澄清的发酵液中纯化γ-氨基丁酸（GABA）。实验比较了凝胶型和大孔树脂特性对EBA工艺性能的影响。结果表明，凝胶型树脂的GABA结合能力高于大孔树脂。这是由于凝胶型树脂具有更好的流体动力学性能和均匀的流动分布。此外，CS16GC有效地去除了≥99%的杂质，GABA产率≥97%。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7484/6472577/209fdc4796c6/CEAT-41-2427-g009.jpg
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