Shahar A, Reuveny S, David Y, Hamdorf G, Terborg M, Cervos-Navarro J
Department of Virology, Israel Institute for Biological Research, Ness-Ziona.
J Biotechnol. 1990 Nov;16(3-4):221-32. doi: 10.1016/0168-1656(90)90038-d.
In stationary cultures of dissociated brain and spinal cord grown on microcarriers (MCs), the neuronal and ependymal cells attached to the MCs forming floating aggregates in which they grow in a three-dimensional pattern. The glial and meningeal elements on the contrary, tend to dissociate from the aggregates and adhere to the plastic dish where they divide to form a monolayer. This different behavior of CNS components is not observed in rotating cultures in which all CNS cells remain attached to the MCs and develop into mature floating structures. This cell separation in stationary MC-cultures which is documented here by SEM and immunocytochemistry, may be useful for analysis and evaluation of the metabolic biochemical events of each of the cellular components derived from the same culture.
在微载体（MCs）上培养的解离脑和脊髓的静止培养物中，神经元和室管膜细胞附着在MCs上，形成漂浮聚集体，它们在其中以三维模式生长。相反，神经胶质和脑膜成分倾向于从聚集体中解离并附着在塑料培养皿上，在那里它们分裂形成单层。在旋转培养物中未观察到中枢神经系统成分的这种不同行为，在旋转培养物中，所有中枢神经系统细胞都保持附着在MCs上并发育成成熟的漂浮结构。本文通过扫描电子显微镜（SEM）和免疫细胞化学记录的静止MC培养物中的这种细胞分离，可能有助于分析和评估来自同一培养物的每种细胞成分的代谢生化事件。
