Favre-Bulle O, Schouten T, Kingma J, Witholt B
Groningen Biotechnology Center, University of Groningen, The Netherlands.
Biotechnology (N Y). 1991 Apr;9(4):367-71. doi: 10.1038/nbt0491-367.
The alk genes from the catabolic OCT plasmid of Pseudomonas oleovorans, which encode the enzymes involved in the oxidation of n-alkanes to carboxylic acids, were introduced into E. coli W3110. The resulting recombinant converts n-octane in a two-liquid phase medium into the corresponding alkanoate and excretes this compound into the aqueous phase. The rate of octanoic acid production by the recombinant E. coli is equal to or better than the alkane oxidation rate of P. oleovorans, suggesting that two-liquid phase fermentations with E. coli might have future industrial applications.
将来自食油假单胞菌分解代谢OCT质粒的alk基因导入大肠杆菌W3110，该基因编码参与将正构烷烃氧化为羧酸的酶。所得重组体在双液相培养基中将正辛烷转化为相应的链烷酸，并将该化合物分泌到水相中。重组大肠杆菌生产辛酸的速率等于或优于食油假单胞菌的烷烃氧化速率，这表明大肠杆菌双液相发酵可能具有未来的工业应用前景。
