Wang S, Foutch G L
School of Chemical Engineering, Oklahoma State University, Stillwater 74078-0537.
Biotechnol Prog. 1991 Mar-Apr;7(2):111-5. doi: 10.1021/bp00008a005.
Reaction rates have been measured for the production of selected bradykinins, angiotensins, and endorphins by solid-phase synthesis. Reactor liquid concentrations are monitored by using a UV detector and flow cell. Experimentation has been limited to low excesses of the t-Boc amino symmetrical anhydride. More than 500 attachments have been monitored. Most data obtained from resins with 1% cross-linking show second-order behavior with reaction rate constants between 0.5 and 8 L/(mol.s). The reaction rate is affected by chemical structures of both the attached amino symmetrical anhydride and the anchored amino terminus on the peptide fragment. Increasing reaction temperature and initial solution concentrations promotes reaction rate. The structure of the polymer support affects not only the reaction rate but also the observed reaction kinetics.
已通过固相合成法测定了特定缓激肽、血管紧张素和内啡肽生成的反应速率。通过使用紫外检测器和流通池监测反应器液体浓度。实验仅限于叔丁氧羰基（t-Boc）氨基对称酸酐的低过量情况。已监测了500多次连接反应。从具有1%交联度的树脂获得的大多数数据显示出二级反应行为，反应速率常数在0.5至8 L/(mol·s)之间。反应速率受连接的氨基对称酸酐和肽片段上锚定氨基末端的化学结构影响。提高反应温度和初始溶液浓度可促进反应速率。聚合物载体的结构不仅影响反应速率，还影响观察到的反应动力学。
