Takeuchi M, Satoh M, Ogura M, Saito H, Takeuchi K
Aichi Cancer Center Hospital, Nagoya.
Res Commun Inst Ferment. 1991(15):22-9.
MEG-01SSF cells were prepared from MEG-01S by conditioning in a chemically defined, serum-free medium. The cells grew well in the medium and required transferrin for growth. Doubling time was about 20 hours. The cells retained GpIIb/IIIa as a marker of megakaryocytes and transferrin receptors on the cell surface. Some of the cells in the stationary phase of growth produced platelet-like particles, which bore characteristic microtubule rings.
MEG - 01SSF细胞由MEG - 01S细胞在化学成分明确的无血清培养基中培养制备而成。这些细胞在该培养基中生长良好，生长需要转铁蛋白。倍增时间约为20小时。细胞保留了作为巨核细胞标志物的糖蛋白IIb/IIIa以及细胞表面的转铁蛋白受体。处于生长静止期的一些细胞产生了带有特征性微管环的血小板样颗粒。
