Takeuchi K, Ogura M, Saito H, Satoh M, Takeuchi M
Ehime College of Health Science, Ehime, Japan.
Exp Cell Res. 1991 Mar;193(1):223-6. doi: 10.1016/0014-4827(91)90560-h.
The distribution of microtubules and platelet-specific glycoproteins (GPIIb/IIIa) in particles was probed by an immunofluorescence method using anti-tubulin and anti-GPIIb/IIIa antibodies to identify whether particles released from a human megakaryoblastic cell line (MEG-01) are platelets. The fluorescence image showing anti-tubulin staining of the particles revealed a characteristic ring structure observed in platelets. Anti-platelet GPIIb/IIIa antibody staining showed an image in which small patches or spots were seen throughout the particle with brighter staining at the periphery. No significant difference was observed between these particles and human blood platelets under immunofluorescent staining. These results show that MEG-01 cells released platelet-like particles.
采用抗微管蛋白抗体和抗血小板糖蛋白IIb/IIIa（GPIIb/IIIa）抗体的免疫荧光法，检测微管和血小板特异性糖蛋白（GPIIb/IIIa）在颗粒中的分布，以确定从人巨核母细胞系（MEG-01）释放的颗粒是否为血小板。显示颗粒抗微管蛋白染色的荧光图像揭示了血小板中观察到的特征性环状结构。抗血小板GPIIb/IIIa抗体染色显示，整个颗粒中可见小斑块或斑点，周边染色更亮。在免疫荧光染色下，这些颗粒与人类血小板之间未观察到显著差异。这些结果表明，MEG-01细胞释放了血小板样颗粒。
