CHARLES A, INGRAM J T
J Biophys Biochem Cytol. 1959 Aug;6(1):41-4. doi: 10.1083/jcb.6.1.41.
Using standard osmium fixation and methacrylate embedding techniques, a study has been made of the melanocyte of human biopsy skin removed under general and local anaesthesia. Melanogenesis was easily observable in the melanocytes, but immature pigment granules were rarely seen in the Malpighian cells. The passage of melanin from melanocyte to Malpighian cell-cytocrine secretion-is thought to have been observed. Phagocytes near the dermal-epidermal junction seem to have their pigment granules in vacuoles, rather than surrounded directly by the cytoplasmic matrix as in the melanocytes. This, together with the failure to observe "effete" melanocytes, prompts the suggestion that the phagocytes are melanocytes which have migrated from the epidermis into the dermis. A melanin granule is shown with alternating dark and lighter transverse striations, concerning which structure little can at present be said.
采用标准的锇固定和甲基丙烯酸酯包埋技术，对在全身麻醉和局部麻醉下切除的人体活检皮肤中的黑素细胞进行了研究。在黑素细胞中很容易观察到黑素生成，但在马尔皮基细胞中很少见到未成熟的色素颗粒。据认为已观察到黑色素从黑素细胞到马尔皮基细胞的传递——细胞分泌。真皮-表皮交界处附近的吞噬细胞似乎将其色素颗粒储存在液泡中，而不像黑素细胞那样直接被细胞质基质包围。这一点，再加上未能观察到“衰老的”黑素细胞，提示吞噬细胞是从表皮迁移到真皮的黑素细胞。图中显示了一个具有交替深色和浅色横纹的黑色素颗粒，目前对其结构知之甚少。
