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从未萌发豌豆胚中分离核仁。

The isolation of nucleoli from ungerminated pea embryos.

作者信息

JOHNSTON F B, SETTERFIELD G, STERN H

出版信息

J Biophys Biochem Cytol. 1959 Aug;6(1):53-6. doi: 10.1083/jcb.6.1.53.

Abstract

A method is described for the isolation of nuclei and nucleoli from ungerminated pea embryos. Electron micrographs of the nucleolar preparation indicate a high degree of purity but only a partial preservation of composition, because of a loss of material from the nucleoli which occurs during the isolation procedure.

摘要

本文描述了一种从未萌发豌豆胚中分离细胞核和核仁的方法。核仁制备物的电子显微镜照片显示其纯度很高,但由于在分离过程中核仁有物质损失,其组成仅得到部分保留。

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The site of ribonucleic acid synthesis in the isolated nucleus.
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本文引用的文献

1
The Isolation of Nuclei and Oxidative Particles from Viable Wheat Embryos.
Plant Physiol. 1957 Mar;32(2):124-9. doi: 10.1104/pp.32.2.124.
2
A particulate component found in nucleoli of Allium cepa and Vicia faba.
J Biophys Biochem Cytol. 1958 Mar 25;4(2):229-30. doi: 10.1083/jcb.4.2.229.
3
Mass isolation of viable wheat embryos.
Nature. 1957 Jan 19;179(4551):160-1. doi: 10.1038/179160b0.

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