JOHNSTON F B, SETTERFIELD G, STERN H
J Biophys Biochem Cytol. 1959 Aug;6(1):53-6. doi: 10.1083/jcb.6.1.53.
A method is described for the isolation of nuclei and nucleoli from ungerminated pea embryos. Electron micrographs of the nucleolar preparation indicate a high degree of purity but only a partial preservation of composition, because of a loss of material from the nucleoli which occurs during the isolation procedure.
本文描述了一种从未萌发豌豆胚中分离细胞核和核仁的方法。核仁制备物的电子显微镜照片显示其纯度很高,但由于在分离过程中核仁有物质损失,其组成仅得到部分保留。