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抗原刺激对分离的淋巴结细胞蛋白质中磷酸盐和蛋氨酸掺入的影响。

The effect of antigenic stimulation on incorporation of phosphate and methionine into proteins of isolated lymph node cells.

作者信息

KERN M, EISEN H N

出版信息

J Exp Med. 1959 Aug 1;110(2):207-19. doi: 10.1084/jem.110.2.207.

DOI:10.1084/jem.110.2.207
PMID:13673135
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2136988/
Abstract

Isolated lymph node cells incorporate inorganic orthophosphate into a protein fraction. The phosphorylated product is a phosphoprotein. The rate of phosphate incorporation into phosphoprotein was determined in cells isolated from regional lymph nodes at varying times after antigen injection. The rate was unaltered on the 3rd day, but was enhanced on the 4th day after injection. Parallel results were obtained with L-methionine incorporation into the same gross protein fraction. Possible relationships between antibody formation and the observed enhancement in phosphate incorporation into phosphoprotein are discussed.

摘要

分离出的淋巴结细胞将无机正磷酸盐掺入一种蛋白质组分中。磷酸化产物是一种磷蛋白。在抗原注射后的不同时间，测定了从局部淋巴结分离出的细胞中磷酸盐掺入磷蛋白的速率。在注射后的第3天，该速率未改变，但在第4天有所增强。将L-甲硫氨酸掺入相同的总蛋白质组分中也得到了类似的结果。文中讨论了抗体形成与观察到的磷酸盐掺入磷蛋白增强之间可能的关系。

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The effect of antigenic stimulation on incorporation of phosphate and methionine into proteins of isolated lymph node cells.抗原刺激对分离的淋巴结细胞蛋白质中磷酸盐和蛋氨酸掺入的影响。
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本文引用的文献

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A study of the distribution of 2,4-dinitrobenzene sensitizers between isolated lymph node cells and extracellular medium in relation to induction of contact skin sensitivity.一项关于2,4-二硝基苯致敏剂在分离的淋巴结细胞与细胞外介质之间的分布与接触性皮肤敏感性诱导关系的研究。
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The transfer of lymph node cells in the study of the immune response to foreign proteins.在对外源蛋白质免疫反应的研究中淋巴结细胞的转移
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Studies of hypersensitivity to low molecular weight substances. III. The 2,4-dinitrophenyl group as a determinant in the preciptin reaction.低分子量物质超敏反应的研究。III. 2,4-二硝基苯基作为沉淀素反应中的一个决定簇
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