KERN M, EISEN H N
J Exp Med. 1959 Aug 1;110(2):207-19. doi: 10.1084/jem.110.2.207.
Isolated lymph node cells incorporate inorganic orthophosphate into a protein fraction. The phosphorylated product is a phosphoprotein. The rate of phosphate incorporation into phosphoprotein was determined in cells isolated from regional lymph nodes at varying times after antigen injection. The rate was unaltered on the 3rd day, but was enhanced on the 4th day after injection. Parallel results were obtained with L-methionine incorporation into the same gross protein fraction. Possible relationships between antibody formation and the observed enhancement in phosphate incorporation into phosphoprotein are discussed.
分离出的淋巴结细胞将无机正磷酸盐掺入一种蛋白质组分中。磷酸化产物是一种磷蛋白。在抗原注射后的不同时间,测定了从局部淋巴结分离出的细胞中磷酸盐掺入磷蛋白的速率。在注射后的第3天,该速率未改变,但在第4天有所增强。将L-甲硫氨酸掺入相同的总蛋白质组分中也得到了类似的结果。文中讨论了抗体形成与观察到的磷酸盐掺入磷蛋白增强之间可能的关系。