HARRIS T N, HARRIS S
J Exp Med. 1949 Aug 1;90(2):169-80. doi: 10.1084/jem.90.2.169.
Following the injection of various antigenic and non-antigenic materials into the foot-pads of rabbits, the draining (popliteal) lymph nodes were removed on successive days and studied histologically, chemically, and serologically. On the 2nd day after injection of antigen, nucleoli and cytoplasmic granules and crescents stained with pyronine began to appear. They were found first in somewhat altered reticulum cells, later in transitional forms, then in young lymphocytes, and finally in more mature lymphocytes. The identity of this pyronine-stained material as ribonucleic acid was demonstrated by specific digestion with protease-free ribonuclease. The concentration of ribonucleic acid was determined in aqueous extracts of the lymph nodes. It was observed that the concentration had risen to more than twice its normal value by the 2nd to 5th day following the injection of antigens into the foot, and then it declined. The peak of this change occurred at or slightly before the appearance of the maximal concentration of antibodies in the same node. Non-antigenic materials, when injected into the foot, did not give rise to an increase in the ribonucleic acid content of the lymph node. The concentration of desoxyribonucleic acid was constant in all lymph nodes, within the limits of experimental variation.
在向兔的足垫注射各种抗原性和非抗原性物质后，连续数天切除引流（腘）淋巴结，并进行组织学、化学和血清学研究。注射抗原后第2天，用派洛宁染色的核仁、细胞质颗粒和月牙形开始出现。它们首先在有些改变的网状细胞中发现，随后在过渡形式中出现，然后在幼淋巴细胞中出现，最后在更成熟的淋巴细胞中出现。通过用无蛋白酶的核糖核酸酶进行特异性消化，证明这种用派洛宁染色的物质是核糖核酸。测定了淋巴结水提取物中核糖核酸的浓度。观察到，在向足部注射抗原后的第2至5天，浓度升至正常水平的两倍以上，然后下降。这种变化的峰值出现在同一淋巴结中抗体最大浓度出现时或稍早于此。当向足部注射非抗原性物质时，淋巴结的核糖核酸含量没有增加。在实验变化范围内，所有淋巴结中的脱氧核糖核酸浓度是恒定的。
